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Molecular Research Center/Methylene Blue Stain (MB 119)/MB 119/500 ml

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Methylene Blue StainMethyleneBlueStainisanaqueoussolutionofmethylenebluedesignedforquantitativeandqualitativeassessmentofRNAandDNAimmobilizedonhybridizationmembranes.ItcanbeusedtoverifytheamountofRNAonhybridizationmembranesinnorthernblotting.Unlikethecarcinogenicagent,ethidiumbromide,MethyleneBlueStaindoesnotintercalateinnucleicacidchainsandaccordingly,doesnotinterferewithnucleicacidretentiononhybridizationmembranesorwiththehybridizationprocess(1).MethyleneBlueStainhasbeentestedandfoundappropriatefornylon/plasticornitrocellulosemembranes,althoughnitrocelluloseexhibitsincreasedbackgroundstaining.MethyleneBlueStainisreusableanddetectsapproximately25ngofRNAorDNAperband.Thestainingprocedureisfast(10min.)andsimple.

References

  1. Maniatis,T.,Fritsch,E.F.andSambrook,J.1982.MolecularCloning.ColdSpringHarborPress.ColdSpringHarbor,NY.
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