TRIReagent®isapatentedreagentfortheisolationoftotalRNAorforthesimultaneousisolationofRNA,DNAandproteins.Thereagentisanimprovedversionofthepopularsingle-stepmethodfortotalRNAisolation(1,2,3).Itisamonophasesolutioncontainingphenolandguanidinethiocyanate.TRIReagent®providesareliable,costeffectiveandefficientmethodofRNAisolation.TRIReagent®allowsforacomprehensiveanalysisofgeneexpressioninavarietyofsamplesofhuman,animal,plant,yeast,bacterialandviralorigin.RNAisolationiscompleteinlessthanonehour,andDNAandproteinisolationsinlessthanthreehours.ThreeversionsofTRIReagent®areavailable,eachdesignedforoptimalisolationefficiencyfromcertaintypesofsamples.
TRIReagent®(TR118)
$68.00–$517.00
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TRIReagent®isusedforRNAisolationfromtissues,pelletedcellsandcellsgrowninmonolayer.Fiftymillilitersissufficienttoprocess50samples,eachcontaining50–100mgoftissue.Expectedyieldsrangefrom50–700μgofRNApersample,dependinguponthetissuesource.
TRIReagent®BD(TB126)
$74.00–$562.00
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TRIReagent®BDisdesignedforusewithwholebloodandplasma.Fiftymillilitersissufficienttoprocess65samplesof0.25mleach.
TRIReagent®LS(TS120)
$74.00–$562.00
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TRIReagent®LSisusedforcellsUSPensionsandotherliquidsamples.Fiftymillilitersissufficienttoprocess65samplesof0.25mleach.
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References
USPatents4,843,155and5,346,994.
- Chomczynski,P.andSacchi,N.Single-stepmethodofRNAisolationbyacidguanidiniumthiocyanatephenol-chloroformextraction.Anal.Biochem.162:156-159.1987.
- Chomczynski,P.Areagentforthesingle-stepsimultaneousisolationofRNA,DNAandproteinsfromcellandtissuesamples.BioTechniques.15:532-537.1993.
- Chomczynski,P.,Bowser-Finn,R.andSabatini,L.Areagentforthesingle-stepisolationofviralRNAfromhumanserumandbiopsysamples.J.NIHRes.6:83.1994.