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Alstembio/HumaniPSCellLine(Episomal,CD34+,ApoE4)/iPS16

HumaniPSCellLine(Episomal,CD34+,ApoE4):#iPS16

ProductDescription

Footprint-freehumaniPS(inducedpluripotentstem)celllinewasderivedfromhumanbonemarrowCD34+mononuclearcellsbyectopicexpressionofOCT4,SOX2,KLF4,c-MYCandLin28genesusingepisomalplasmids.ThecellswerederivedusingmorphologicalselectioncriteriaandwithouttheuseoffluorescentMarkerordrugselection.WhenculturedunderstandardhumanEScellcultureconditions,themorphologyofhumaniPScellsareidenticaltothatofhumanEScells.ThecellsalsoexpressthepluripotencymarkersTRA-1-60,SSEA-3andOct4,anddemonstratestrongendogenousalkalinephosphataseactivity.
 
Figure1.CharacterizationofiPSCsderivedfromhumanbonemarrowCD34+mononuclearcells.ImmunostainingofhiPSCcoloniesexpressingESCspecificmarkersOCT4,Nanog,andTRA-1-60.
 
Figure2.Thecellshaveanormalkaryotype.
 
Neuralrosette(ectoderm)Bone(mesoderm)Glandularstructure(endoderm)
Figure3.Invivopluripotencytestbyteratomaformation.Variouscelltypessuchasneuralrosette(ectoderm),bone(mesoderm),andglandularstructures(endoderm)arefound.
 
ProductSpecifications
ProductNameHumaniPSCellLine(Episomal,CD34+,ApoE4)
Catalog#iPS16
Size>2x105cells/vial
ShippingDryIce
StorageandStABIlityStoreingasphaseofliquidnitrogenimmediatelyuponreceipt.Thisproductisstablefor6monthswhenstoredasdirected.
QualityControlHumaniPScellsweregrowninfeederfreeconditionswithmTeSR1medium.EachlotofhumaniPScellsistestedforgrowthandviabilityfollowingrecoveryfromcryopreservation.Inaddition,eachlotistestedforexpressionofTRA-1-60andOct4,aswellastheactivityofalkalinephosphatase.
SafetyPrecautionALSTEMhighlyrecommendsthatprotectivegloves,alabcoat,andafull-facemaskalwaysbewornwhenhandlingfrozenvials.Itisimportanttonotethatsomeliquidnitrogencanleakintothevialswhensubmersedinliquidnitrogen.Uponthawing,theliquidnitrogenreturnstothegasphase,resultinginexcessivepressurewithinthevialthatcancausethevialtoexplodeorexpelthecapwithdangerousforce.
RestrictedUseForResearchUseOnly.Notforuseindiagnosticortherapeuticprocedures.
ProtocolDownload
  • Overview
  • Procedure
ThisprotocolcanbeusedforculturinghumaniPScells.Footprint-freehumaniPScellsweregeneratedbytransientlyintroducingepisomalplasmidsencodingthehumantranscriptionfactorsintohumanperipheralbloodmononuclearcells.Thecellswerederivedusingmorphologicalselectioncriteriaandwithouttheuseoffluorescentmarkersordrugselection.WhenculturedunderstandardhumanEScellcultureconditions,themorphologyoffootprint-freehumaniPScellsisidenticaltothatofhumanEScells.ThecellsalsoexpressthepluripotencymarkersTRA-1-60andOct4,anddemonstrateastrongendogenousAPactivity.
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