CatalogNo.:AB0100-200
Price/Unit:EUR300.00
Source:Goat
Quantity/unitsize:600µg
Description:GoatpolyclonalantibodytoCD45.Itisa180-220kDatypeItransmembraneglycoproteinandamemberoftheproteintyrosinephosphatase(PTP)family.ThisPTPcontainsanextracellulardomain,asingletransmembranesegmentandtwotandemintracytoplasmiccatalyticdomains,andthusisclassifiedasareceptortypePTP.Itisexpressedinallhematopoieticcells,buthighestonlymphocytes.PTPsareknowntobesignallingmoleculesthatregulateavarietyofcellularprocessesincludingcellgrowth,differentiation,mitosis,andoncogenictransformation.IthasintrinsictyrosinephosphataseactivityanditisessentialforTandBcellantigenreceptor-mediatedactivation.Itfunctionsthrougheitherdirectinteractionwithcomponentsoftheantigenreceptorcomplexes,orbyactivatingvariousSrcfamilykinasesrequiredfortheantigenreceptorsignalling.CD45alsosuppressesJAKkinases,andthusfunctionsasaregulatorofcytokinereceptorsignalling.Thecross-linkingofCD45inducesapoptosisinlymphocytes.Alternativenames:B220,CD45antigen,CD45R,GP180,L-CA,LCA,leukocytecommonantigen,LY5,proteintyrosinephosphatase,PTPRC,receptortypecpolypeptide,receptor-typetyrosine-proteinphosphataseC,RP11-553K8.4,T200,T200glycoprotein,T200leukocytecommonantigenantibody.
Form:Polyclonalantibodysuppliedasa200µl(3mg/ml)aliquotinPBS,20%glyceroland0.05%sodiumazide.Thisantibodyisepitope-affinitypurifiedfromgoatantiserum.
Immunogen:Purifiedrecombinantpeptidederivedfromwithinresidues1,260aatotheC-terminusofhumanCD45producedinE.coli.
Specificity:UsingJurkatcelllysatedetectsaband180-220kDabyWesternblot.
Reactivity:Reactsagainsthuman,rat,mouse,canineandmonkeyproteins.
Usage:Westernblot 1:500-1:2,000Immunofluorescence NDImmunohistochemistry(paraffin) NDImmunohistochemistry(frozen) ND
Reactivitychart
Sample | WesternBlot | Immuno-fluorescence | Immuno-Histochemistry(p) | Immuno-Histochemistry(f) | human | +++ | ND | ND | ND | rat | +++ | ND | ND | ND | mouse | +++ | ND | ND | ND | canine | +++ | ND | ND | ND | monkey | +++ | ND | ND | ND |
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Storage:Storeat-20Cforlong-termstorage.Storeat2-8Cforuptoonemonth.
Specialinstructions:Avoidfreeze/thawcycles.
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