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| 服务名称： | AgilentaCGH芯片检测 |
AgilentaCGH芯片检测
简介
拷贝数变异（包括DNA拷贝数增加（重复）、丢失（缺失）以及多等位基因或复杂重排）是基因组变异的重要原因之一，它与人类的很多疾病相关，如孟德尔遗传疾病；智力缺陷；孤独症；精神分裂症；肿瘤；自身免疫病等等。传统的染色体核型分析方法（如染色体区带染色分析，FISH等）可以检测染色体大范围结构变异。但这些方法具有实验操作繁琐，分辨率低，不能覆盖全基因组等缺点。基于基因芯片的比较基因组杂交技术（array-basedComparativeGenomicHybridization,aCGH）可以更加准确地发现疾病相关的染色体的变异，为疾病的诊断和治疗提供极大的帮助，这对高风险人群的健康水平筛查，疾病预防有重要意义。Agilent利用其先进的芯片生产工艺，开发出高性能的aCGH芯片，广泛用于基因的拷贝数检测和染色体结构变化检测。AgilentCGH芯片覆盖全基因组，在包括基因区域（内含子区、外显子区）、基因间区域以及对疾病研究极其重要的亚端粒区域（但不包含重复序列）的分布大致相同。
实验流程
技术优势
灵敏度和特异性：相比其它CGH芯片平台，具有极高的灵敏度和特异性，可有效检测单拷贝的失和扩增；
适用样品类型广：能够有效检测FFPE（Formalin-FixedandParrffin-Embedded，FFPE）样品；
数据处理软件强大：AgilentGenomicWorkbench具有图形化显示，强大的数据统计功能；
可有效检测肿瘤发生过程中伴随的DNA拷贝数变化：如染色体的畸变；寻找新的原癌基因或抑癌基因；发现新的DNA拷贝数标记，用于临床诊断、疾病的分型等。
产品列表
物种 | 产品名称 | 产品编号/设计ID | 芯片格式 | 探针数量 | 探针间距(中位值) | 数据库版本 |
人Human | SurePrintG3HumanCGHMicroarray1×1M | G4447A/021529 | 1x1M | 963,029 | 2.1KBoverallmedian;1.8KBinRefseqgenes | UCSChg18(NCBIBuild36),March2006 |
SurePrintG3HumanCGHMicroarray2x400K | G4448A/021850 | 2x400K | 411,056 | 5.3KBoverallmedian;4.6KBinRefseqgenes | UCSChg18(NCBIBuild36),March2006 | |
SurePrintG3HumanCGHMicroarray4x180K | G4449A/022060 | 4x180K | 170,334 | 13KBoverallmedian;11KBinRefseqgenes | UCSChg18(NCBIBuild36),March2006 | |
SurePrintG3HumanCGHMicroarray8×60K | G4450A/021924 | 8x60K | 55,077 | 41KBoverallmedian;33KBinRefseqgenes | UCSChg18(NCBIBuild36),March2006 | |
SurePrintHDHumanCGHMicroarray1x244K | G4411B/014693 | 1x244K | 236,381 | 8.9KBoverallmedian;7.4KBinRefseqgenes | UCSChg18(NCBIBuild36),March2006 | |
SurePrintHDHumanCGHMicroarray2×105K | G4412A/014698 | 2x105K | 99,026 | 22KBoverallmedian;19KBinRefseqgenes | UCSChg18(NCBIBuild36),March2006 | |
SurePrintHDHumanCGHMicroarray4x44K | G4426B/014950 | 4x44K | 42,494 | 43KBoverallmedian;24KBinRefseqgenes | UCSChg18(NCBIBuild36),March2006 | |
小鼠Mouse | SurePrintG3MouseCGHMicroarray1x1M | G4838A/027414 | 1x1M | 963,261 | 1.8KBoverallmedian;1.5KBinRefseqgenes | UCSCmm9(NCBIBuild37),July2007 |
SurePrintG3MouseCGHMicroarray4x180K | G4839A/027411 | 4x180K | 170,305 | 10.9KBoverallmedian;9.1KBinRefseqgenes | UCSCmm9(NCBIBuild37),July2007 | |
SurePrintHDMouseCGHMicroarray1x244K | G4415A/014695 | 1x244K | 235,402 | 7.8KBoverallmedian;6.2KBinRefseqgenes | UCSCmm8(NCBIBuild36),February2006 | |
SurePrintHDMouseCGHMicroarray2x105K | G4416A/014699 | 2x105K | 98,797 | 19.3KBoverallmedian;15.7KBinRefseqgenes | UCSCmm8(NCBIBuild36),February2006 | |
大鼠Rat | SurePrintG3RatCGHMicroarray1×1M | G4840A/027065 | 1x1M | 962,967 | 1.7KBoverallmedian,1.3KBinRefseqgenes | UCSCrn4(BaylorHGSCBuild3.4)November2004 |
SurePrintG3RatCGHMicroarray4x180K | G4841A/027064 | 4x180K | 170,012 | 10.7KBoverallmedian,8.0KBinRefseqgenes | UCSCrn4(BaylorHGSCBuild3.4)November2004 | |
SurePrintHDRatCGHMicroarray1x244K | G4435A/015223 | 1x244K | 235,974 | 4.9KBoverallmedian,4.7KBinRefseqgenes | UCSCrn4(BaylorHGSCBuild3.4),November2004 | |
SurePrintHDRatCGHMicroarray2x105K | G4436A/015235 | 2x105K | 97,973 | 19.1KBoverallmedian,11.3KBinRefseqgenes | UCSCrn4(BaylorHGSCBuild3.4),November2004 | |
牛Bovine | SurePrintG3BovineCGHMicroarray4×180K | G4826A/025242 | 4x180K | 171,527 | 11.0KBoverallmedian | UCSCbosTau4(October2007) |
犬Canine | SurePrintG3CanineCGHMicroarray4×180K | G4826A/025522 | 4x180K | 171,534 | 2.7kboverallmedian | UCSCcanFam2(May2006) |
鸡Chicken | SurePrintHDChickenCGHMicroarray1x244K | G4423B/019553 | 1x244K | 235,980 | 4KBoverallmedian | UCSCgalGal3(WUSTLBuild2.1)May2006) |
黑猩猩Chimpanzee | SurePrintG3ChimpanzeeCGHMicroarray4×180K | G4826A/024422 | 4x180K | 171,524 | 12.7kboverallmedian | UCSCpanTro2(March2006) |
水稻Rice | SurePrintG3RiceCGHMicroarray4×180K | G4826A/025843 | 4x180K | 171,861 | 14.9kboverallmedian | RGSPBuild5(June2008) |
恒河猴RhesusMacaque | SurePrintG3RhesusMacaqueCGH4×180K | G4826A/025843 | 4x180K | 171,495 | 14.9kboverallmedian | UCSCrheMac2(January2006) |