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InnovaBiosciences’ProteinA–HorseradishPeroxidaseconjugatesaremanufacturedusingourLightning-Link®antibodylabelingtechnology,thereforeprovidingextremelyhighquality,stableconjugates.Foraddedflexibilityweprovidetheconjugatesintwodifferentpacksizesof100ugand1mg.Furthermoretheconjugatesarefreeze-driedsoshippingatambienttemperatureisnotaproblem.
ProteinAhasahighaffinityfortheFcregionsofIgGmoleculesfromavarietyofspecies.ImmobilizationofproteinAcreatesanaffinityresinthatcanbeusedtoisolateIgGfractionsfromcrudeserum,ascitesfluidorhybridomasupernatants.AliquotsofProteinAresinmayalsobeusedtopulldownantibody:antigencomplexesinimmunoprecipitationexperiments.ProteinAresinispreparedbycouplinghighlypurifiedproteinAtoagarosebeads.
HorseradishPeroxidase(HRP)isametalloenzymewhichhasmanydifferentisoforms.Itutiliseshydrogenperoxidetooxidizebothorganicandinorganiccompounds,andinthepresenceoftheappropriatesubstrate(e.g.TMB,DAB,ABTS)causesacolorchangethatcanbemeasuredspectrophotometrically.
FluorescenceintensityofInnovaBiosciences’ProteinA–HorseradishPeroxidaseconjugateversuscompetitor
Figure1.AProteinA–HorseradishPeroxidaseconjugatewaspurchasedfromacommercialsupplier.TheconjugatewascomparedtoProteinA–HorseradishPeroxidasefromInnovaBiosciencesinanELISA.ItcanbeseenthattheInnovaBiosciencesproteinA–HorseradishPeroxidaseconjugateoutperformsthecompetitor.