5columnsfordelglycosylationof5x0.5mgofIgG.
ThecolumncontainsGlycINATOR(EndoS2)enzymeimmobilizedonagarose.GlycINATORisanendoglycosidasethatrapidlycleavestheN-glycanstructureoftheFcdomainofIgGs.TheN-glycaniscleavedafterthefirstGlcNAc.
GlycINATOReffectivelyremoveshigh-mannoseaswellascomplexandhybridFc-glycansonnativeIgGs.