BCIP5-Bromo-4-chloro-3-indolylphosphatedisodiumsalt102185-33-1100MG￥693.00SIGMAB6149
BCIP5-Bromo-4-chloro-3-indolylphosphatedisodiumsalt102185-33-1500MG￥2,259.00SIGMAB6149
BCIP5-Bromo-4-chloro-3-indolylphosphatedisodiumsalt102185-33-11G￥3,686.00SIGMAB6149
BCIP5-Bromo-4-chloro-3-indolylphosphatedisodiumsalt102185-33-15G￥14,780.00SIGMAB6149
产品名称BCIP：5-Bromo-4-chloro-3-indolylphosphatedisodiumsalt——【产品编号：SIGMA-B6149
Forthecolorimetricdetectionofalkalinephosphatase-labeledmolecules,5-Bromo-4-chloro-3-indolylphosphate(BCIP)andNitroBlueTetrazolium(NBT)areavailable.TheBCIP/NBTsubstratesystemisversatileandfunctionsinavarietyofapplications,includingNorthernSouthern,andWesternblotting,insituhybridization,andimmunohistochemistry.
BCIPisprovidedintwosaltforms:thedisodiumsaltwhichissolubleinwaterandthep-toluidineformwhichissolubleindimethylformamide.ThesesaltformsmaybeusedtoprepareastocksolutionthatincombinationwithNBTandareactionbuffer,formasubstratesolutionforalkalinephosphatase.Thissubstratesystem,whenincubatedwithalkalinephosphatase,producesaninsolubleNBTdiformazanproductthatiseasilyobservablewithitspurplecolor.Foraddedconvenience,amixtureofBCIPandNBTisprovidedinaneasilydissolvabletabletformorasaready-to-useliquid.法律信息BCIPisaregisteredtrademarkofSigma-AldrichCo.LLC
参考文献
McGadey,J.,Atetrazoliummethodfornon-specificalkalinephosphatase.Histochemie23,180-184,(1970)
Horwitz,J.P.,etal.J.Med.Chem.9,447,(1966)Leary,J.J.,etal.,Rapidandsensitivecolorimetricmethodforvisualizingbiotin-labeledDNAprobeshybridizedtoDNAorRNAimmobilizedonnitrocellulose:Bio-blots.Proc.Natl.Acad.Sci.U.S.A.80,4045-4049,(1983)Knecht,D.A.,Dimond,R.L.,VisualizationofantigenicproteinsonWesternblots.Anal.Biochem.136,180,(1984)Walters,C.,etal.,DetectionofparvovirusB19inmaceratedfetaltissueusinginsituhybridization.J.Clin.Pathol.50(9),749-754,(1997)Meltzer,J.C.,etal.,Enhancedimmunohistochemicaldetectionofautonomicnervefibers,cytokinesandinduciblenitricoxidesynthasebylightandfluorescentmicroscopyinratspleen.J.Histochem.Cytochem.45(4),599-610,(1997)
