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celexplorer/FocusClear™/500 ml/11520

Introduction
FocusClear™ is non-toxic, ready to use and a water-soluble clearing agent for enhancing the transparency of cross-linking agent-fixed biological specimens and its clearing effect is able to reach up to a depth of 500 microns below the specimen surface. With the use of FocusClear™ to significantly increase the effectiveness of laser excitation and detection of color and fluorescence, obtained microscopic image quality will be greatly improved. Samples labeled with fluorescence and non-fluorescence dyes including lipophilic dyes, such as DiI, DiD and NBD-ceramide can be directly transferred from water, buffer solutions, alcohol, DMSO, DMF, and glycerin into FocusClear™ solution.
Highlights
  • A water-soluble clearing agent that increases the transparency of biological tissues.
  • Allows for visualization of internal objects up to 500 μm below the tissue surface.
  • Increases depth of view by at least 2X over traditional glycerol-based mounting media.
  • Increases the efficacy of laser excitation and optical signal detection of either color or fluorescence.
  • Suitable for immunofluorescent-labeled single cells and tissues, in situ hybridization, tissue or whole-mount immunohistochemistry and fluorescent protein samples.

Product Specifications

Specification

Refractive Index: 1.45

pH: 11

Application
Animal: brain, kidney, heart, stomach, intestine, liver, lung, pancreas, skinInsect: brain, ventral nerve cord, ovaryBiomaterial: collagen matrixPlant
Clearing
Solitary use
Recommended0.5~1mm depth tissue slice
With CLARITY*
Recommended Whole organ
With SCALEVIEW**
Not recommended
ClearingSpeed
Solitary use
Within 1 Hour
With CLARITY
Days
With SCALEVIEW
-
Immunostaining Compatibility
Yes
Fluorescent protein Compatibility
Yes
Organic dye Compatibility
Yes
Lipophilic dye Compatibility
Yes
Objective lens
Objective lens with high NA and long WD is suggested,Leica HCX IRAPOL 25X water-immersion objective, NA 0.95, WD 2.4mm;Zeiss 20X W-PlanApochromat objective, NA 1.0, WD 2.0 mmOlympus XLSLPLN25XSVMP objective, NA 0.9, WD 8.0 mm
Cat. No.
FC-101
FC-102
FC-103
Format
5ml
50ml
500ml
Storage
4~3039~86

MSDS for FocusClear

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