Altogen/293 Transfection Reagent (Emb Kidney, CRL-1573)/1.5 ml CRISPR(Catalog #2204)

Description

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TransfectionReagentfor293Cells(EmbryonicKidneyCells,CRL-1573)

• Twocomponentformulationenhanceslipidmediatedtransfectionefficiency
• Optimizedeasy-to-usetransfectionprotocolprovidedfortransfectionofsiRNA,DNA,mRNA,andmicroRNA
• KitincludesTransfectionEnhancerreagentandrecommendedtransfectionprotocol
• Hightransfectionefficacyinthe presenceofserum
• ExpandyourRNAiapplicationwithareagentoptimizedfordeliveryofbothsiRNAandplasmid
• ReproducIBLetransfectionresults
• Workswellforstandardreversetransfectionandhigh-throughputapplications
• Downloadinvitro293transfectionprotocol:[PDF]
• Download 293CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationfor293 cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:
Reagentexhibitsatleast90%transfectionefficiencyofsiRNAdelivery.Transfectionefficiencywasdeterminedbyreal-timeRT-PCR.

TransfectionProtocolandMSDS:
DownloadAltogenBiosystems293TransfectionProtocol:[PDF]
DownloadMSDS:[PDF]

293CellLine:
The293celllinewasestablishedfromembryonicandtumOrigenickidneycellsofahumanfetus.Thiscelllineexpressesvitronectinreceptorscomposedoftheintegrinbeta-1subunitaswellasthevitronectinreceptoralpha-vsubunitandcouldbeusefulfortestingefficacyofvirucide.Thiscelllinealsomakesanexcellenttransfectionhost.The293celllineisahypotriploidhumancelllinethatexhibitsepithelialmorphology.Themodalchromosomenumberof64occursnearlyin30%ofcells.293cellscontainAdenovirus5DNAontheleftendsequenceoftheviralgenome.Allother293typesofcelllinesarederivativesofthisparentcellline.Allofthedaughtercellsexhibitsimilarcharacteristics.The293celllinewasderivedfromcancerousfetalkidneytissueandisareliablehostfortransfectionstudiescarriedoutforcancerresearch.AltogenBiosystemsprovidestransfectionkitswithlowcytotoxicityforthe293cellline.

Data:

Figure1.GAPDmRNAlevelswerequantifiedusingreal-timeRT-PCRinthe293 cellstransfectedwithsiRNAstargetingGAPDornon-silencingsiRNA.Forty-eighthourspost-transfection,thecellswereharvestedandanalyzedbyreal-timeRT-PCRforGAPDmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=5).

Figure2.ProteinexpressionofGAPDHin293cells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedinto293cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):

• Nature.2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]
• Am JPathology.2010177(4):1870-80.RoleofocularcomplementfactorHinamurinemodel…Lyzogubovetal[PDF]
• NatureBiotechnology.201129(4):341-5.DeliveryofsiRNAtothemousebrainby…Alvarez-Ervitietal [PDF]
• CancerResearch.201171(15):5144-53.InhibitionofmiR-193aexpressionby…Iliopoulosetal[PDF]
• Hypertension.2015 65(2):430-9.Neurokinin3receptorandphosphocholinetransferase…Parchimetal [PDF]

AltogenBiosystems:

AltogenBiosystemsisalifesciencescompany thatmanufacturesover100celltypespecificandpreoptimizedtransfection kits, elecroporationbuffers,andtargetedinvivo deliverykitsandreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovidehighlyefficientintracellulardeliveryofbiomolecules(proteins,DNA,mRNA,shRNA andsiRNA,andsmallmoleculecompounds).Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.

AltogenResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#1151)
• 1.5ml(Catalog#1152)
• 1.5mlCRISPR(Catalog#2204)
• 8.0ml(Catalog#7014)