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TransfectionReagentfor 3LLCells(LewisLungCarcinoma Cells)
TransfectionEfficiency:
Reagentexhibitsatleast73%transfectionefficiencyofsiRNAdelivery.Transfectionefficiencywasdeterminedbyreal-timeRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystems3LLTransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
3LLCellLine:
Lungcancerisconsideredthemostpreventablemalignancyglobally.However,itisthedeadliestformofcancerwithveryhighmortalityratesbecauseoflackofadequatetherapiesandaggressivetumorcellgrowth.Rodentcelllinemodelsplayanessentialroleinpreclinicalresearchtostudynoveltherapeuticagentsandtreatinghumancancer.TheLLCcelllineisawell-studiedmousecancermodelthatisextensivelyemployedasatransplantablemalignancymodel.LewisLungCarcinomacells(alsoknownas3LLcellsorLLCcells)gottheirnameafterDr.MargaretR.LewisoftheWistarInstitute,whodiscoveredcarcinomain1951.Thiscelllinehasbeenknowntoshowreducedmetastaticabilitywhenexposedtoultravioletlight.Ithasalsobeenobservedthatthepresenceofcannabinoidssuppressescellgrowth.Thesecellscouldbeusefulformedicalresearchfortreatmentstocertaintypesoflungcancer,andmanyothermolecularorcellBIOLOGystudies.Accordingtotherecentdata,theproliferativeadvantageof3LLmetastaticcellsisachievedbythegeneexpressionwhichpreventstumorcellsfromescapinghostilemicroenvironmentalconditions.AltogenBiosystemsprovidesnanoparticle-basedtransfectionkitsforthiscellline.
Data:
Figure1.GAPDmRNAlevelswerequantifiedusingreal-timeRT-PCRinthecellstransfectedwithsiRNAstargetingGAPDornon-silencingsiRNA.Forty-eighthourspost-transfection, 3LLcellswereharvestedandanalyzedbyreal-timeRT-PCRforGAPDHmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=3).
Figure2.ProteinexpressionofGAPDHin3LLcells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedinto3LLcellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):
AltogenBiosystems:
AltogenBiosystemsmanufacturers celltypespecificandpreoptimizedtransfectionproducts, elecroporationkits,andinvivodeliveryreagentsforlifescienceresearch.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofprotein,DNA,mRNA,shRNA andsiRNAmolecules.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.
AltogenResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
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