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Altogen/3LL Transfection Reagent (Lewis Lung Carcinoma)/1.5 ml(Catalog #1702)

Description

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TransfectionReagentfor 3LLCells(LewisLungCarcinoma Cells)

  • Ananoparticle-basedliposomeformulation
  • Transfectionprotocolsprovidedfortransfectionofproteins,DNA,mRNA,siRNA,shRNAandmicroRNA
  • TransfectionEnhancerreagentprovidedwiththekit
  • Producehigherlevelofrecombinantproteinexpressionwithminimaldisruptionofnormalcellfunction
  • Generatephysiologicallyrelevantdatayoucantrust
  • EffectiveforplasmidDNA/siRNAco-transfection
  • Lowcytotoxicity
  • Downloadinvitro3LLtransfectionprotocol:[PDF]
  • Download 3LLCRISPR/Cas9transfectionprotocol:[PDF]
  • DownloadPowerPointpresentationfor3LLcellstransfectionkit:[PPT]
  • DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:
Reagentexhibitsatleast73%transfectionefficiencyofsiRNAdelivery.Transfectionefficiencywasdeterminedbyreal-timeRT-PCR.

TransfectionProtocolandMSDS:
DownloadAltogenBiosystems3LLTransfectionProtocol:[PDF]
DownloadMSDS:[PDF]

3LLCellLine:
Lungcancerisconsideredthemostpreventablemalignancyglobally.However,itisthedeadliestformofcancerwithveryhighmortalityratesbecauseoflackofadequatetherapiesandaggressivetumorcellgrowth.Rodentcelllinemodelsplayanessentialroleinpreclinicalresearchtostudynoveltherapeuticagentsandtreatinghumancancer.TheLLCcelllineisawell-studiedmousecancermodelthatisextensivelyemployedasatransplantablemalignancymodel.LewisLungCarcinomacells(alsoknownas3LLcellsorLLCcells)gottheirnameafterDr.MargaretR.LewisoftheWistarInstitute,whodiscoveredcarcinomain1951.Thiscelllinehasbeenknowntoshowreducedmetastaticabilitywhenexposedtoultravioletlight.Ithasalsobeenobservedthatthepresenceofcannabinoidssuppressescellgrowth.Thesecellscouldbeusefulformedicalresearchfortreatmentstocertaintypesoflungcancer,andmanyothermolecularorcellBIOLOGystudies.Accordingtotherecentdata,theproliferativeadvantageof3LLmetastaticcellsisachievedbythegeneexpressionwhichpreventstumorcellsfromescapinghostilemicroenvironmentalconditions.AltogenBiosystemsprovidesnanoparticle-basedtransfectionkitsforthiscellline.

Data:

3LL cell transfection kit

Figure1.GAPDmRNAlevelswerequantifiedusingreal-timeRT-PCRinthecellstransfectedwithsiRNAstargetingGAPDornon-silencingsiRNA.Forty-eighthourspost-transfection, 3LLcellswereharvestedandanalyzedbyreal-timeRT-PCRforGAPDHmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=3).

3LL-cells-transfection-protocol

Figure2.ProteinexpressionofGAPDHin3LLcells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedinto3LLcellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):

  • Nature.2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]
  • NatureBiotechnology.201129(4):341-5.DeliveryofsiRNAtothemousebrainby…Alvarez-Ervitietal [PDF]
  • RNA.201016(11):2108-19.RNaseLreleasesasmallRNAfromHCVRNAthatrefolds…Malathietal[PDF]
  • Diabetologia.201255(7):2069-79.Thep47phox-andNADPHoxidaseorganiser1…Younetal [PDF]
  • Circulation Research.201015;107(8).Kruppel-likefactor-4transcriptionallyregulates…Cowanetal[PDF]
  • Hypertension.201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]
  • JounalofBiologicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
  • PLoSPathogens.20128(8)Uridinecompositionofthepoly-U/UCtractofHCVRNA…Schnelletal[PDF]
  • JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]

RequestaQuotefor
3LLTransfectionReagent

AltogenBiosystems:
AltogenBiosystemsmanufacturers celltypespecificandpreoptimizedtransfectionproducts, elecroporationkits,andinvivodeliveryreagentsforlifescienceresearch.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofprotein,DNA,mRNA,shRNA andsiRNAmolecules.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

  • 0.5ml(Catalog#1701)
  • 1.5ml(Catalog#1702)
  • 1.5mlCRISPR(Catalog#2206)
  • 8.0ml(Catalog#7015)
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