Altogen/IMR-90 Transfection Kit (Lung Fibroblast, CCL186)/8.0 ml(Catalog #6832)

Description

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TransfectionReagentforIMR-90Cells(Lung Fibroblast Cells,CCL-186)

• Twocomponentformulationenhanceslipidmediatedtransfectionefficiency
• KitincludesTransfectionEnhancerreagentandrecommendedtransfectionprotocol
• ExpandyourRNAiapplicationwithareagentoptimizedfordeliveryofbothsiRNAandplasmid
• ReproducIBLetransfectionresults
• Workswellforstandardreversetransfectionandhigh-throughputapplications
• DownloadinvitroIMR-90transfectionprotocol:[PDF]
• Download IMR-90CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforIMR-90cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast91%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsIMR90TransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

IMR-90CellLine:

Fibroblastsarethemostcommonconnectivetissuecellspresentinthebodythatareresponsibleformakingupthestructuralframeworksincetheyproducetheextracellularmatrixandcollagen.Fibroblastsareelongatedcellswitharoundorovalnucleusthatcanbeseenintheinterstitialspacesoforgans.Activefibroblastsareusuallylargerandcontainabundantroughendoplasmicreticulum.Inthelung,theyaretypicallysituatedinhighlycomplexmulticellularenvironments.Abetterunderstandingofdifferentfibroblastphenotypescouldacceleratethepathtodiscoveryoftargetedtherapeutics.

TheIMR-90celllinewasestablishedin1975fromlungfibroblastsofa16-week-oldCaucasianfemalefetusbyW.W.NicholsandassociatesandisasuitabletransfectionhostforavarietyofresearchrelatedtomolecularBIOLOGy.TheIMR-90celllinedisplayscharacteristicsofbothfibroblastsanddifferentiatedsmoothmusclecellsandcanreachroughly58populationdoublingsbeforetheonsetofsenescence(aging).Thesecellsarehighlyutilizedasmodelfibroblastsinabroadarrayofstudies.Atthesametime,theyexhibitvariouscharacteristicsofdifferentiatedsmoothmusclecells,includingthesameelongatedformaswellasthelongitudinalalignmentoftheiractin-attachmentsites.AltogenBiosystemsprovidestwo-componentshighlyefficienttransfectionreagentkitsforthiscellline.

Data:

Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRintheIMR-90cellstransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=6).

Figure2.ProteinexpressionofCyclophilinBinIMR-90cells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedintoIMR-90cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:
AltogenBiosystemsprovidespre-optimizedtransfectionkitsandelectroporationproductsforlifescienceresearch.Transfectionproductsaredevelopedforindividualcancercelllineandtransfectionprotocolsareoptimizedtoenablehightransfection efficiencyofbiomolecules.AltogenBiosystemsdevelopedinvivodeliveryproductsforsmallanimalresearch,mouseandrattargetedtissuedelivery:livertargeted,pancreastargeted,kidneytargeted,PEG-Liposome-,Nanoparticle-,Lipid-,andPolymer-basedinvivotransfectionkits.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofbiomolecules.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#6830)
• 1.5ml(Catalog#6831)
• 1.5mlCRISPR(Catalog#2160)
• 8.0ml(Catalog#6832)