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WholeCellLysate,1mg(2mg/ml)
GeneralNotes:
Growthmedia:DMEMsupplementedwith10%FBS
CelllysatewaspreparedbyhomogenizationinRIPAbuffer(25mMTrisHClpH7.6,150mMNaCl,1%NP-40,1%sodiumdeoxycholate,0.1%SDS)supplementedwith5µg/mlofaprotininand5µg/mlofleupeptin.Celldebriswereremovedbycentrifugation(5minutesat12,000RPM).ConcentrationoftotalproteinwasdeterminedusingBCAproteinassay.
StorageInstructions
Shippedat4°C.Upondeliveryaliquot.Storeat-80°C.Avoidfreeze/thawcycle.
Volume
0.5ml
Concentration
1mgat2mg/ml
Purity
WholeCellLysate
AltogenLabsResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
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