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| CatalogNumber: 21202 |
| GeneSymbol: ERBB2/HER2/Neu |
| Description: Anti-ErbB2RabbitPolyclonalAntibody |
| Background: TheErbBfamilyiscomposedoffourplasmamembrane-boundreceptortyrosinekinases.Amplificationorover-expressionoftheERBB2geneoccursinapproximately30%ofbreastcancers.HER2isencodedbyERBB2,aknownproto-oncogenelocatedatthelongarmofhumanchromosome17(17q21-q22).HER2proteinshavebeenshowntoformclustersincellmembranesthatmayplayaroleintumOrigenesis. |
| Immunogen: AsyntheticpeptidefromthecentralofErbB2protein. |
| Applications: ELISA,WB,IHC |
| RecommendedDilutions: ELISA:1:500-1:5000WB:1:200-1:2000IHC:1:20-1:200 |
| Concentration: 0.2mg/ml |
| Host: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Isotype: |
| Purity: Purifiedfromserum |
| Storagebuffer: |
| Preservative: no |
| Constituents: PBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,50%glycerol |
| SpeciesReactivity: RecognizesErbB2ofvertebrates. |
| StorageConditions: Storeat-20°C.Avoidfreeze/thawcycles. |
DownloadDatasheet:  |
| Westernblot: |
 | WesternblotanalysisofErbB2protein.PurifiedfulllengthHis-taggedErbB2protein(Cat.#10208)wasblottedwithanti-ErbB2rabbitpolyclonalantibody(Cat.#21202). |
 | Immunohistochemicalanalysisofparaffin-embeddedhumanbreastcancertissuewithanti-ERBB2polyclonalantibody(Cat.#21202).Tissuesampleswerefixedwithformaldehydeandblockedwith1%serumfor15minat37°C.Antigenretrievalwasbyheatmediationincitratebuffer(pH6).Sampleswerethenincubatedwithprimaryantibody(1:50dilution)overnightat4°C.AHRP-conjugatedGoatanti-RabbitIgG(1:50)wasusedassecondaryantibody. |
| RelatedProducts | |||||
| Catalog# | Name | Size | Price | DataSheet | |
| 21200 | Anti-ERBB2RabbitpolyclonalAntibody | 100μL | $219 |  | |
| 21202 | Anti-ERBB2RabbitPolyclonalAntibody | 100μL | $219 | | |
| 26080 | Anti-ERBB2(V777A)mousemonoclonalAntibody | 100μL | $495 | | |
| 26127 | Anti-ERBB2mousemonoclonalAntibody | 100μL | $239 | | |
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