细菌的核酸抽提

DNAExtraction

·DNAExtractionfromBacteria(JulieB.Wolf,UMBC)Phenol/chloroformmethod

·DNAExtractionFromBacteria(TritonMethod)(NWFSC)

• IsolationofChromosomalDNAfromBacteria(HancockLab)

·RapidGenomicDNAIsolationfromP.Aeruginosa(GoldbergLab)

·DNAExtractionfromGram-positiveBacteria(NWFSC)ThisprocedurewasoriginallydevelopedforListeriamonocytogenesbuthasworkedwellwithotherGram+bacteria

·GenomicDNAisolationfromBacteria(NewmanLab)

·DNAExtractionfromBacteria(LazoLab)Phenol/chloroformmethod

·GenomicDNAIsolation(JeffreyD.Newman)Phenol/chloroformmethod,thisproceduredoesnotworkwellwithGram+cocci

• IsolationofAspergillusDNA(MolecularGeneticsNetworkLab)
• IsolationofAspergillusDNA:QUIAGENMethod(MolecularGeneticsNetworkLab)

·IsolationandQuantificationofGenomicDNAfromMycobacteriumtuberculosis(TheNortheastRegionalRFLPLaboratoryforDNATyping)Thisprotocoldescribesa"DNAmini-prep"procedurefortheisolationofchromosomalDNAfromMycobacteriumtuberculosisandaprocedureforquantifyingtheamountisolated

RNAExtraction

·IsolationOfRNAFromBacteroidsandBacteria(LazoLab)

• ExtractionofRNAfromAspergillus:-Guanidin-thiocyanateandCsClmethod(MolecularGeneticsNetworkLab)
• ExtractionofRNAfromAspergillus:-Guanidin-thiocyanateandLiClmethod(MolecularGeneticsNetworkLab)

Others

·Pulsed-fieldGelPurificationofE.coliRestrictionFragments(StanfordDNASequence&TechnologyCenter)