The Chaperone Plasmid Set consists offive different plasmids, each of which is designed to express multiple molecular chaperones that function together as a "chaperone team" to enable optimal protein expression andfolding and to reduce protein misfolding. Co-expression of a target protein with one of these five chaperoneplasmids increases the recovery of soluble protein and minimizes product loss as a result of insoluble protein. Each plasmid carries an origin of replication (ori)derived from pACYC and a chloramphenicol-resistance gene (Cmr) gene, which allows theuseof E. coli expression systemscontaining ColE1-type plasmids that confer ampicillin resistance. The chaperone genes are situated downstream ofthe araB orPzt-1 (tet) promoters; as a result, expression of target proteins and chaperones can be individually induced if the target gene is placed under the control ofdifferent promoters (e.g., lac). These plasmids also contain the necessary regulator (araC or tetr) for each promoter.
The Chaperone Plasmid Set consists offive different plasmids, each of which is designed to express multiple molecular chaperones that function together as a "chaperone team" to enable optimal protein expression andfolding and to reduce protein misfolding. Co-expression of a target protein with one of these five chaperoneplasmids increases the recovery of soluble protein and minimizes product loss as a result of insoluble protein. Each plasmid carries an origin of replication (ori)derived from pACYC and a chloramphenicol-resistance gene (Cmr) gene, which allows theuseof E. coli expression systemscontaining ColE1-type plasmids that confer ampicillin resistance. The chaperone genes are situated downstream ofthe araB orPzt-1 (tet) promoters; as a result, expression of target proteins and chaperones can be individually induced if the target gene is placed under the control ofdifferent promoters (e.g., lac). These plasmids also contain the necessary regulator (araC or tetr) for each promoter.
Plasmids in the Chaperone Plasmid Set work well in combination withthe pCold Expression System Vectors.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn