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| ProductName | Cat# | Clone# | Isotype | Applications | Reactivity | Unit/Size |
| AntiHumanCOUP-TFI | PP-H8132-00 | H8132 | IgG2a | EL,WB,IP,IHC | Hu,Mo,Ra | 100ug/0.1ml |
*Immunohistochemistry
Immunohistochemistry:HumanProstateglandparaffinsection
*Description
ChickenovalbuminupstreampromotertranscriptionfactorI(COUP-TFI,EAR3,COUP-TFA;NR2F1)isamemberoforphannuclearreceptor.COUP-TFIisexpressedinspecificregionsoftherostralbrain,instripesinthepresumptivehindbrain.COUP-TFIhasvariedrolesinthedevelopmentoftheperipheralnervoussystem,suchasearlyregionalizationoftheneocortex,differentiationofsubplateneuronsandguidanceofthalamocorticalaxons.COUP-TFswereshowntointeractwithanumberofothernuclearreceptors.
SpecSheetPP-H8132-00(PDFFile)
| Alias | Chickenovalbuminupstreampromotertranscriptionfactor1,COUP-TF?,V-ERBArelatedproteinEAR-3 |
| GeNEBank | X12795 |
| Nomenclature | NR2F1 |
| Origin | ProducedinBALB/cmouseascitesafterinoculationwithhybridomaofmousemyelomacells(NS-1)andspleencellsderivedfromaBALB/cmouseimmunizedwithBaculovirus-expressedrecombinanthumanCOUP-TFI(6-81aa). |
| Specificity | ThisantibodyspecificallyrecognizeshumanCOUPTFIandcrossreactswithmouseandratCOUP-TFI.ThisantibodydoesnotrecognizehumanCOUP-TFIIandEAR2. |
| Purification | Ammoniumsulfatefractionation |
| Formulation | Physiologicalsalinewith0.1%NaN3asapreservative. |
| Storage | Storeat2C-8Cuptoonemonth.Forlong-termstorage,thesolutionmaybefrozeninworkingaliquots.Repeatedfreezingandthawingisnotrecommended.Storageinafrost-freefreezerisnotrecommended. |
| Reference | SuhJM,etal.MolEndocrinol.2006;20(12):3412-20QinJ,etal.DevDyn.2007;236(3):810-20PerilhouA,etal.MolCellBiol.2008;28(14):4588-97 |
| Note | Sodiumazidemayreactwithleadandcopperplumbingtoformexplosivemetalazides.Flushwithlargeamountsofwaterduringdisposal. |
Cautionmustbetakentoavoidcontactwithskinoreyes.Insuchacase,rinsethoroughlyatoncewithwater.Donotingest,inhale,orswallow.Seekmedicalattentionimmediately.Wearappropriateprotectiveclothingsuchaslaboratoryoveralls,safetyglassesandgloves.Itisstronglyadvisedthatthisproductshouldbehandledbypeoplewhohavebeenwelltrainedinlaboratorytechniquesandthatitishandledwithcarepursuanttotheprinciplesofgoodlaboratorypractice.Allchemicalsaredeemedpotentiallyharmful.Thevialispronetofallover.Usecaution,especiallywhenthelidisoff.
FORRESEARCHUSEONLY,NOTFORUSEINDIAGNOSTICPROCEDURES.
*Remark-Icon:
EL=ELISA,WB=WesternBlotting,NW=Non-reducingWesternBlotting,S=SuperShiftAssay.IP=Immunoprecipitation,IHC=Immunohistochemistory,CH=ChIPAssay
Hu=Human,Mo=Mouse,Ra=Rat
Manufacturedby:PerseusProteomics,Inc.
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