Iwai-Chem/GAPDH Monoclonal Antibody/200ul/E-AB-20079-200ul

Description

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation. 

Spec Sheet E-AB-20079 (PDF File)

Synonyms	GAPDH,GAPD,CDABP0047,OK/SW-cl.12,Glyceraldehyde-3-phosphate dehydrogenase,GAPDH,Peptidyl-cysteine S-nitrosylase GAPDH
Swiss-Prot	P04406
Concentration	1 mg/mL
Host	Mouse
Immunogen	Synthetic Peptide
Purification Method	Antigen affinity purification
Formulation	PBS with 0.02% sodium azide, 50% glycerol, PH7.4
Storage	Store at -20?. Avoid freeze / thaw cycles.
Dilution	WB 1:500-1:2000

Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off .

FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.

* Remark Icon :

WB=Western Blotting, IP=Immunoprecipitation, IF=Immunofluorescence, IHC=Immunohistochemistory, IHC-p=Immunohistochemistory Paraffin, FCM=Flow Cytometry, CH=ChIP Assay

Manufactured by:Elabscience