Paratechs 昆虫细胞系 VEBEVS Insect Cell Lines Nitrate Reductor

Paratechs

Nitrate Reductor Kit Paratechs

The Device can be used to analyze nitrate as nitrite in physiological fluids using a single disposable microplate for both the reduction process and the Griess reaction colorimetric assay. The Reductor has broad application in biomedical research involving in vivo nitric oxide production.

The Device also has application in agricultural research, soil testing, and environmental monitoring.

Expensive, single-use nitrate analysis kits are replaced with a reusable device suitable for analysis of several thousand samples.

Laboratories that routinely perform ELISA testing procedures should already have the necessary equipment and supplies, and would only need to purchase the Reductor to acquire the capability to perform nitrate analysis more efficiently than with virtually any other analytical system.

A video demonstrating the use of the nitrate reductor can be found below.

Nitrate Reductor Kit Paratechs
Product number: 50010		Price: $395

Uses

• Nitrate may be successfully analyzed in virtually any sample matrix.
• The Standard Additions Method protocol described in the cited publication, allows the relationship of water standards to statistically derived sample concentrations to be accurately established in a single operation. An assessment of the absolute accuracy of the method for a given matrix can thus be easily determined.

Advantages

• Expenses are reduced from several dollars per analysis when using other commercially available nitrate analysis kits to only a few cents when using the ParaTechs Reductor.
• The need for expensive, dedicated instrumentation such as continuous-flow analyzers is eliminated. Very little expertise, beyond accurate pipetting skills, is required to perform the analysis.
• The Reductor has no moving parts and the analysis requires no start-up time. The reagents are simple to prepare and are stable for extended periods.
• Using multiple Reductors, the daily laboratory throughput for nitrate analysis is limited only by the number of individuals applied to the task.

VE-BEVS Insect Cell Lines Paratechs

•VE-CL-01 Product No. 10010 •VE-CL-02 Product No. 10020 •VE-CL-03 Product No. 10030

Improved protein expression from the baculovirus expression vector system (BEVS) utilizing ParaTechs’ Vankyrin-Enhanced (VE) Sf9 insect cells. Further enhancement by using VE cells with our VE-transfer vector.

• Engineered monoclonal Sf9 cell line stably expresses a vankyrin gene

• Up to 14-fold increase in protein yield as compared to parental Sf9 cells

• Prolonged longevity of cells after infection with a BEVS

• Compatible with all conventional BEVS

• Reduced cost and labor

• Essentially no additional work or adaptation required

ParaTechs offers three VE-insect cell lines for retail. Each of which has slightly different characteristics to help to enhance the expression of different types of recombinant protein.

VE-CL-01 cells Paratechs
Product number: 10010	Unit: 107cells/ml	Price: $800

VE-CL-01 cell line

Sf9-derived cell line for the enhanced expression of highly stable intracellular and secreted proteins

This cell line offers a much prolonged expression time that greatlyenhances the yield of stable proteins. These cells are suitable for the expression of highly stable intracellular and secreted proteins. The cells are sold in batches of 1×107/ml cells in serum-free medium. Please note this product ships on dry ice so additional shipping charges may apply.

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VE-CL-02 cells Paratechs
Product number: 10020	Unit: 107cells/ml	Price: $800

VE-CL-02 cell line

Sf9-derived cell line for the enhanced expression of highly unstable or toxic proteins.

This cell line is recommended for laboratories working with highly unstable or toxic proteins as enhanced protein expression occurs at a very high level and over a very short time interval.

The cells are sold in batches of 1×107/ml cells in serum-free medium. Please note this product ships on dry ice so additional shipping charges may apply.

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VE-CL-03 cells Paratechs
Product number: 10030	Unit: 107cells/ml	Price: $800

VE-CL-03 cell line

Sf9-derived cell line for general enhancement of most recombinant proteins.

This cell line is recommended for the enhanced expression of proteins of unknown toxicity and stability or for general enhancement of most recombinant proteins expressed in conventional recombinant BEVS viruses.

The cells are sold in batches of 1×107/ml cells in serum-free medium. Please note this product ships on dry ice so additional shipping charges apply.

VE-BEVS Cell Line Instructions

VE-BEVS FAQs

VE-BEVS Licensing

Contract service for introducing the vankyrin gene into a custom insect cell line is available upon request.

VE-BEVS Transfer Vectors Paratechs

•pAcVE1 Product No. 20010 •pAcVE.02 Product No. 20020 •pAcVE.03 Product No. 20030

Enhanced recombinant protein production in insect cells when utilizing ParaTechs’ Vankyrin-Enhanced (VE) transfer vectors. Recombinant protein production is further enhanced when used in combination with our VE-insect cells.

• Expression of gene of interest from polyhedron promoter

• Co-expression of vankyrin gene from p10 promoter

ParaTechs offers three VE-BEVS Transfer Vectors for retail, each is a baculovirus transfer vector designed for high levels of recombinant gene expression under the control of the Autographa californica nucleopolyhedro virus (AcMNPV) polyhedrin promoter.

Transfer Vector pAcVE1 Paratechs
Product number: 20010	Unit: 10µg	Price: $350

pAcVE1

This baculovirus transfer vector integrates the co-expression of a vankyrin gene for increased protein production.

Click here to view/download the: Transfer Vector pAcVE1 [PDF]

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Transfer Vector pAcVE.02 Paratechs
Product number: 20020	Unit: 10µg	Price: $350

pAcVE.02

This baculovirus transfer vector integrates the co-expression of a vankyrin gene for increased protein production with

• honey bee melittin signal sequence for direct secretion and
• N-terminal 8x His-tag for ease of purification

Click here to view/download the: Transfer Vector pAcVE.02 [PDF]

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Transfer Vector pAcVE.03 Paratechs
Product number: 20030	Unit: 10µg	Price: $350

pAcVE.03

This baculovirus transfer vector integrates the co-expression of a vankyrin gene for increased protein production with

• honey bee melittin (HBM) signal for direct secretion and
• optional C-terminal 6x His-tag for ease of purification

Click here to view/download the: Transfer Vector pAcVE.03 [PDF]

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VE-BEVS Transfer Vector Resources

• pAcVE1 Instructions [PDF]
• pAcVE.02 Instructions [PDF]
• pAcVE.03 Instructions [PDF]

VE-BEVS Frequently Asked Questions [PDF]

VE-BEVS Licensing

NSET

ParaTechs’ NSET device revolutionizes mouse embryo transfer by reducing costs and eliminates the need for animal anesthesia and recovery normally required for conventional surgical procedures.

VE-BEVS

ParaTechs’ Vankyrin-Enhanced Baculovirus Expression Vector System or VE-BEVS technology enhances protein expression from the BEVS while reducing the cost of labor and materials.

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