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reprocell/Sox2 mRNA, Human/05-0016-base

Stemgent Sox2 mRNA encodes the Sox2 protein commonly used in cellular reprogramming systems for the generation of induced pluripotent stem (iPS) cells. This in vitro transcribed mRNA incorporates both pseudouridine and 5-methylcytidine modified nucleotides to minimize the cellular interferon response to single stranded RNA.1,2Repeated transfection of Sox2 mRNA in combination with mRNAs encoding Oct4, Klf4, Lin-28 and c-Myc has been shown to reprogram human fibroblasts.2

Figure 1: Protein expression and localization shown by immunocytochemistry, DAPI in blue and Sox2 in red.

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