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reprocell/Oct4 mRNA, Human/05-0014-base

Stemgent Oct4 mRNA encodes the Oct4 protein commonly used in cellular reprogramming systems for the generation of induced pluripotent stem (iPS) cells. This in vitro transcribed mRNA incorporates both pseudouridine and 5-methylcytidine modified nucleotides to minimize the cellular interferon response to single stranded RNA.1,2 Repeated transfection of Oct4 mRNA in combination with mRNAs encoding Klf4, Sox2, Lin-28 and c-Myc has been shown to reprogram human fibroblasts.2

Figure 1: Protein expression and localization shown by immunocytochemistry, DAPI in blue and Oct4 in red.

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