ScriptCap™ Cap 1 Capping System | CSCCS1710 | CELLSCRIPT_价格厂家供应商_蚂蚁淘（苏州）生物科技有限公司_蚂蚁淘

：蚂蚁淘（苏州）生物科技有限公司

The ScriptCap™ Cap 1 Capping System provides both ScriptCap Capping Enzyme and ScriptCap 2'-OMethyltransferase allowing the user to produce either a Cap 0 or Cap 1 cap structure on the 5' end of in vitro transcribed (IVT) RNA. ScriptCap Capping Enzyme catalyzes in vitro addition of a cap nucleotide to the 5' terminus of primary RNA that has a 5'-triphosphate group. A cap nucleotide or “cap” is a guanine nucleoside that is joined via its 5' carbon to a triphosphate group that is, in turn, joined to the 5' carbon of the most 5' nucleotide of the primary mRNA transcript, and in most eukaryotes, the nitrogen at the 7 position of the guanine in the cap nucleotide is methylated. Such a capped transcript can be represented as: m7G(5')ppp(5')N1(pN)x-OH(3') where m7G represents the N7-methylguanosine cap nucleoside, ppp represents the triphosphate bridge between the 5' carbons of the cap nucleoside and the first nucleotide of the primary RNA transcript, and N1(pN)x-OH(3') represents the primary RNA transcript, of which N1 is the most 5' nucleotide.
The ScriptCap Capping Enzyme sequentially catalyzes three different enzymatic reactions:
(1) RNA triphosphatase cleaves the 5' triphosphate of RNA to a diphosphate.pppN1(p)Nx-OH(3') ppN1(pN)x-OH(3') + Pi
(2) RNA guanyltransferase joins GTP to the 5' diphosphate of the most 5' nucleotide (N1) of the RNA.ppN1(pN)x-OH(3') + GTP G(5')ppp(5')N1(pN)x-OH(3') + PPi
(3) Guanine-7-methyltransferase, using S-adenosyl-methionine (SAM or AdoMet) as a co-factor,catalyzes methylation of the 7-nitrogen of guanine in the cap nucleotide.
G(5')ppp(5')N1(pN)x-OH(3') + AdoMet m7G(5')ppp(5')N1(pN)x-OH(3') + AdoHyc This process, referred to as “capping,” improves the stability and translation efficiency of the RNA compared to uncapped RNA. The capped RNA product built by ScriptCap Capping Enzyme has a “cap 0” structure. Cap 0 RNA can be converted to a cap 1 structure in vitro by the simultaneous use of ScriptCap 2'-O-Methyltransferase in the capping reaction together with the ScriptCap Capping Enzyme. ScriptCap 2'-O-Methyltransferase prepares Cap 1-RNA from Cap 0-RNA by transferring a methyl group from the donor molecule SAM to the 2'-O position of the penultimate nucleotide of a cap 0 RNA to synthesize RNA with a cap 1 structure. m7GpppN1(pN)x-OH(3') + AdoMet m7Gppp[m2’-O]N1(pN)x-OH(3') + AdoHyc Cap 0 RNA Cap 1 RNA Cap 1 RNA can further increase in vivo translation efficiency of the mRNA as well as to help mark the mRNA as self RNA relative to intracellular immuno-surveillance mechanisms. A standard ScriptCap Cap 1 Capping System reaction will cap approximately 60 µg of RNA, but reactions can be scaled up or down to accommodate the user’s needs. Capped RNA from an ScriptCap Cap 1 Capping System reaction can be added directly to an A-Plus™ Poly(A) Polymerase reaction (CELLSCRIPT), without prior cleanup, for poly(A)-tailing of the 3' ends of the capped RNA allowing for convenient synthesis of mRNA. The ScriptCap Cap 1 Capping System offers an alternative to making capped RNA by co-transcriptional capping during an IVT reaction in which a dinucleotide cap analog (e.g., m7GpppG) is included in place of a portion of the GTP.3 Provided that the 5' terminus of the RNA is not structured, the capping efficiency using the ScriptCap Cap 1 Capping System can approach 100%. In contrast, since the cap analog competes with GTP for initiation of transcription by the RNA polymerase, co-transcriptional capping efficiency is limited by the concentration of the cap analog and the ratio of its concentration to that of the GTP. Thus, the percentage of RNA that is capped using a cap analog in a transcription reaction is always less than 100%.4,5 The amount of capped RNA that can be made in a co-transcriptional capping reaction using a cap analog is also limited by the need to reduce GTP concentrations to permit the cap analog to compete for initiation of transcription. On the other hand, co-transcriptional capping with a cap analog can be beneficial if the RNA to be capped has a highly structured 5' terminus. Contact CELLSCRIPT to discuss the options for your project. The ScriptCap Cap 1 Capping System improves upon co-transcriptional capping methods by ensuring virtually 100% transcript capping, all caps in the proper orientation and the ability to produce large amounts of capped RNA at a reasonable cost.

CELLSCRIPT C-SCCS1710 品牌名称：
CELLSCRIPT

CELLSCRIPT C-SCCS1710 品牌logo：

CELLSCRIPT C-SCCS1710 品牌官网：
http://www.cellscript.com

CELLSCRIPT C-SCCS1710 品牌介绍： CELLSCRIPT专注于再生医学，细胞再生学以及细胞疗法。细胞、组织或器官的移植治疗及修复中应用的细胞或生物体。CellScript还生产用于传递和翻译的合成修饰mRNA。

The mission of CELLSCRIPT™ is to provide the best products and technologies for making and using RNA for translation in cells for clinical research and therapeutics.

Current products include kits for in vitro transcription, 5' RNA capping using either a cap analog or capping enzymes, and 3' RNA polyadenylation, as well as all-in-one kits for making capped, poly(A)-tailed mRNA for translation in cells.

CELLSCRIPT™ recently introduced INCOGNITO™ RNA Transcription Kits for in vitrosynthesis of RNA that contains modified nucleosides, such as pseudouridine (Ψ) and/or 5-methylcytidine (m5C) in place of the corresponding U or C canonical nucleosides (Figure 1). These kits are so-named because the capped, polyadenylated and nucleoside-modified RNA products (called INCOGNITO mRNAs ) are disguised so they do not induce innate immune responses to the same extent as the corresponding unmodified mRNAs when transfected into mammalian cells that express a variety of RNA sensors.
No. 品牌货号名称规格 1 CELLSCRIPT C-ACM04037 AmpliCap-Max™ T7 High Yield Message Maker Kit 25 rxns 2 CELLSCRIPT C-AC0706 AmpliCap™ SP6 High Yield Message Maker Kit 25 rxns 3 CELLSCRIPT C-C50110 Anti-Reverse Cap Analog (ARCA) 500 nmol 4 CELLSCRIPT C-CC15011H Cap-Clip™ Acid Pyrophosphatase 100 U 5 CELLSCRIPT C-ICSY110510 INCOGNITO™ SP6 Ψ-RNA Transcription Kit 10 rxns 6 CELLSCRIPT C-MSC100625 T7 mScript™ Standard mRNA Production System 25 rxns 7 CELLSCRIPT C-MMA60710 MessageMAX™ T7 ARCA-Capped Message Transcription Kit 10 rxns 8 CELLSCRIPT C-SRI6310K ScriptGuard™ RNase Inhibitor 10,000 U 9 CELLSCRIPT C-SCMT0610 ScriptCap™ 2'-O-Methyltransferase Kit 10 rxns 10 CELLSCRIPT C-ICTY110510 INCOGNITO™ T7 Ψ-RNA Transcription Kit 10 rxns 11 CELLSCRIPT C-MMA60710 MessageMAX™ T7 ARCA-Capped Message Transcription Kit 10 rxns 12 CELLSCRIPT C-C61025 Monomethylated RNA Cap Analog 1,250 nmol 13 CELLSCRIPT C-PRM101125 PseudoU-RNA Molecular Weight Markers 25 lanes 14 CELLSCRIPT C-AS3106 SP6-Scribe™ Standard RNA IVT Kit 50 rxns 15 CELLSCRIPT C-PAP5104H A-Plus™ Poly(A) Polymerase Tailing Kit 50 rxns
CELLSCRIPT C-SCCS1710 联系方式：
联系人：谢先生
400电话：4009658633
办公电话：010-56256916
QQ：3212874516,3198592576
网址：http://www.annoron.com/

CELLSCRIPT C-SCCS1710 产品相关链接：
1 ADP P/N 384

2 Collagen P/N 385

3 Thrombin P/N 386

4 Arachidonic Acid P/N 390

5 Epinephrine P/N 393

供应商：蚂蚁淘（苏州）生物科技有限公司

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