JPT/EBV Dextramer Package 1APC/RX05APC/

EBV Dextramer Package 1-APC

Product Code: RX05-APC

Package of 4 carefully selected EBV specific MHC I Dextramers (+ 1 control), for sensitive and specific detection of antigen-specific T cells using flow cytometry. Each mulitmer is APC labeled.
Amount: 5 x 50 tests
Application(s): Cellular immune response, Immune monitoring, T-cell assays
Indication(s)/Topic(s): Infection, Cancer, Hodgkins lymphoma, Burkitts lymphoma, Nasopharyngeal carcinoma, Dermatomyositis, Systemic lupus erythematosus, Rheumatoid arthritis, Sjgrens syndrome, Multiple sclerosis
Delivery Time: 10-12 days

MHC I Dextramer Collections
MHC I Dextramers combine a dextran polymer backbone with a high number of MHC and fluorochrome molecules providing mulitple binding domains for antigen-specific T cell receptor binding. This enhances their staining intensity and increases resolution as well as signal-to-noise ratio with minimal background staining. In addition, the dextran backbone stabilizes the MHC-peptide complexes and fluorochromes. The EBV set is useful for enumeration of EBV-specific CD8+ T cells, e.g. for immune monitoring in immune-compromised individuals following transplantation, testing of virus-specific vaccines or profiling of virus-specific T cell responses. The Dextramers are provided as APC conjugates.

The following 5 Dextramers are provided with APC label

Dextramer: HLA-A*0201 / GLCTLVAML - Antigen: BMLF-1
Dextramer: HLA-B*3501 / HPVGEADYFEY - Antigen: EBNA-1
Dextramer: HLA-B*0702 / RPPIFIRRL - Antigen: EBNA-3A
Dextramer: HLA-B*0801 / FLRGRAYGL - Antigen: EBNA-3A
Dextramer: General / Negative Control

Benefits of MHC I Dextramers®
- More MHC molecules and more fluorochromes than conventional MHC multimers
- Higher sensitivity (important for low-affinity epitopes as commonly found in cancer & autoimmune disease)
- High stability
- Custom-made Dextramers available upon request

EBV Dextramer Package 1-APC

Selected References for MHC I Dextramers®
"Combination Immunotherapy after ASCT for Multiple Myeloma Using MAGE-A3/Poly-ICLC Immunizations Followed by Adoptive Transfer of Vaccine-Primed and Costimulated Autologous T Cells"
Rapoport et al., Clin Cancer Res (2014) - PMID: 24520093
"Molecular Imprint of Exposure to Naturally Occurring Genetic Variants of Human Cytomegalovirus on the T cell Repertoire"
Smith et al., Sci Rep (2014) - PMID: 24509977
"Probing the effector and suppressive functions of human T cell subsets using antigen-specific engineered T cell receptors"
Wan et al., PLoS One (2013) Full text
"Human Natural Killer Cells Prevent Infectious Mononucleosis Features by Targeting Lytic Epstein-Barr Virus Infection"
Chijioke et al., Cell Rep (2013) - PMID: 24360958