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| Description | This Cas9 Double Mutant (dCas9) has changes at amino acid positions D10A and H840A which completely inactivate both the nuclease and nickase activities. With a target-specific guide RNA (sgRNA), it can bind to a specific region of DNA without cutting it. This vector provides an in-frame downstream multiple cloning site, allowing users to attach any protein of interest to the C-terminus of dCas9. The resulting construct will also carry a C-terminal nuclear localization signal. |
|---|---|
| SKU | K014 |
| System | Lentiviral Vector |
| Vector Map | pLenti-EF1a-dCas9-Cloning |
| Unit quantity | 10 µg |
| Promoter | EF1a |
| Cas Type | Null Mutant |
| Cas Origin | spCas9 |
| Cas9 Expression | Constitutive Cas9 |
| Shipping Conditions | Shipped at ambient temperature |
| Bacterial Selection | Kanamycin |
| Format | Vector |
| Appearance | Liquid |
| Quality Control | Restriction Enzyme Digest and Sequencing |
| Shelf Life | 1 year (when stored at -20°C or below) |
| Caution | This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |
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