EagleBio/iLite FGF-21 Assay Ready Cells/BM3071

iLite FGF-21 Assay Ready Cells

iLite FGF-21 Assay Ready Cells Developed and Manufactured by Svar Life Science

The Eagle Biosciences iLite® FGF-21 Assay Ready Cells are intended for :

• Testing FGF-21 functional activity
• Testing NAb against FGF-21

Receipt and Storage: Upon receipt confirm that adequate dry-ice is present and the cells are frozen. Immediately transfer to -80°C storage. Cells should be stored at -80°C (do not store at any other temperature) and are stable as supplied until the expiry date shown. Cells should be used within 30 min of thawing and should be diluted immediately after thawing.
For Research Use Only

Key benefits of iLite® Assays

• Highly specific reporter gene cell lines
• Very sensitive cell line responses (>10 fold inductions)
• Assay Ready Cells – ready-to-use from the freezer, without culturing of cells
• Assays within a workday (typically 4-7 hour assays)
• Normalization gene, which eliminates unwanted matrix effects

Background

Human fibroblast growth factor 21 (FGF21) is a member of a family of the atypical fibroblast growth factors that include FGF19 and FGF23 in man. FGF21 lacks the heparin-binding domain of conventional FGFs and can consequently diffuse throughout the body and function as a hormone. FGF21 stimulates glucose uptake in adipocytes and the effects on glucose uptake is additive with insulin (1). The iLite® FGF21 ARCs are a genetically engineered reporter gene cell line responsive to FGF21 by specific and proportional expression of Firefly Luciferase. The receptor chain FGFR1c is overexpressed on the surface together with the co-factor β-Klotho which has been genetically optimized to enhance sensitivity. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.

Products Related to FGF-21 Assay Ready Cells

Total FGF-21 ELISA Assay
cTerminal FGF21 ELISA Kit
N-Terminal FGF21 ELISA Kit

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