Viagenbiotech/Proteinase K Solution 5ml (20mg/ml)/501PK/1 Ea

QuickOverview:

GenomicPCRGradeProteinaseKSolution 

CatalogNo.501-PK

Stablefor12monthsat-20℃/ShipsRefrigeratedFedexOvernight

ProteinaseKisanendolyticproteasethatcleavespeptidebondsatthecarboxylicsidesofaliphatic,aromaticorhydrophobicaminoacids.ProteinaseKisclassifiedasaserineprotease.Thesmallestpeptidetobehydrolysedisatetrapeptide.

DNAExtractionlysisreagentforDNAtests&genotyping:TheProteinaseKsolutionisusefulwhenasmallamountofbiologicalsamplesareprocessed.AddtheenzymesolutiontoDirectPCRreagentsatthefinalconcentrationof0.5-1.0mg/ml(25-50μlProteinaseKsolutionper1mlDirectPCRreagent).FollowtheinstructionmanualsforDirectPCRReagentProtocol/Manualforeachblood,bloodstain,buccalswab,hairrootDNAgenotyping.OncedilutedinDirectPCRreagents,ProteinaseKisstablefor~24hrs.

Otherapplications:ProteinaseKdigestsnativeproteinsveryeffectively.ItcanthereforebeusedtoremoveDNasesandRNaseswhenisolatingDNAandRNAfromtissuesorcells.

UsageConditions:

•ProteinaseKisactiveoverawidepHrange—optimalactivitybetween6.5and9.5

•Underdenaturingconditions—e.g.,inthepresenceofSDSorurea

•Inthepresenceofmetalchelatingagents—e.g.,EDTA

•Athightemperatures—optimumdigestiontemperatureis65°C

UnitDefinition:Oneunitoftheenzymeliberatesfolin-positiveaminoacidsandpeptidescorrespondingto1μmoltyrosinein1minat37°Cusingdenaturedhemoglobinassubstrate.

QualityControl:Testedfortheabsenceofendo-,exodeoxyribonucleasesandribonucleases