CatalogNo:202-Y
Forusewithyolksac
Stableupto12monthsat4℃
ShipsatRT
ViagenDirectPCR®DNAExtractionSystemisasingle-tubesystemforrapidpreparationofDNAfrommousetails,earpieces,yolksac,andculturecells.Thepatent-pendingcomponentsdevelopedbyscientistsatViagenBiotechInc.allowtheresultingDNAextractstobecompatiblewithgenomicPCRforgenotyping.Crudeextractsofbiologicalsamplesarenotcompatiblewithmanymolecularbiology-gradereactionssuchaspolymerasechainreaction(PCR),inpartduetoinhibitorscontainedincrudeextracts.TheDirectPCRreagentsnotonlymediatetherapidlysisofbiologicalsamplesbutalsocontaininhibitorsthateffectivelysuppresstheinhibitoryactivitiesofcrudelysatesforPCRamplification,whilemaximallymaintainingtheintegrityofreleasedgenomicDNA.Thepatent-pendingsimpleprocedurecompletelyeliminateanysolutiontransferortube-openingsteps,providingyouwithsubstancialextratime.
Briefprocedure
1. LysetailsinDirectPCR®LysisReagent.
2. Incubatefor45minat85°C.
3. PCRgenotypingwith1μllysates.
Detailedprotocols: Tail,Ear,YolkSac,andCulturedcells.
DirectPCR®systemoffersadvantagesoverconventionalprotocolsthatinclude:
· Timesaving: Lesshands-ontime.CrudetaillysatesforPCR.
· Moneysaving: Cost-effectivereactions.
· Safe:Noorganicreagents.
· Environmental: Lesswaste(organicreagents,tubes,tips,etc...)
· Reliableandefficient:Virtually100%successratewithhighyields.
DirectPCRLysisReagent(yolksac)
201-Y50mlyolksacs
202-Y100mlyolksacs