产品资料

RayBiotech/Human RTK Phosphorylation Array C1/2 Sample Kit/AAH-PRTK-1-2

Antigen Information

Gene Symbols:

ABL1 | AXL | BLK | BMX | BTK | CSF1R | CSK | DDR2 | EGFR | EPHA1 View more Hide

Assay Format

Pathway:

PI3K-AKT Signaling

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
ABL1 Gene Symbol: ABL1 Gene ID: 25 Accession #: P00519	ACK Gene Symbol: TNK2 Gene ID: 10188 Accession #: Q07912 ELISA Kit: RayBio Human Phosphotyrosine ACK ELISA	ALK-1 Gene Symbol: ALK Gene ID: 238 Accession #: Q9UM73 ELISA Kit: RayBio Human Phosphotyrosine ALK ELISA	Axl Gene Symbol: Axl Gene ID: 558 Accession #: P30530 ELISA Kit: RayBio Human Phosphotyrosine AXL ELISA	Blk Gene Symbol: BLK Gene ID: 640 Accession #: P51451 ELISA Kit: RayBio Human Phosphotyrosine BLK ELISA
BMX Gene Symbol: BMX Gene ID: 660 Accession #: P51813 ELISA Kit: RayBio Human Phosphotyrosine BMX ELISA	Btk Gene Symbol: BTK Gene ID: 695 Accession #: Q06187 ELISA Kit: RayBio Human Phosphotyrosine BTK ELISA	Csk Gene Symbol: CSK Gene ID: 1445 Accession #: P41240 ELISA Kit: RayBio Human Phosphotyrosine CSK ELISA	Dtk Gene Symbol: TYRO3 Gene ID: 7301 Accession #: Q06418 ELISA Kit: RayBio Human Phosphotyrosine DTK ELISA	EGFR Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533 ELISA Kit: RayBio Human Phosphotyrosine EGFR ELISA
EphA1 Gene Symbol: EphA1 Gene ID: 2041 Accession #: P21709 ELISA Kit: RayBio Human Phosphotyrosine EphA1 ELISA	EphA2 Gene Symbol: EphA2 Gene ID: 1969 Accession #: P29317 ELISA Kit: RayBio Human Phosphotyrosine EphA2 ELISA	EphA3 Gene Symbol: EphA3 Gene ID: 2042 Accession #: P29320 ELISA Kit: RayBio Human Phosphotyrosine EphA3 ELISA	EphA4 Gene Symbol: EphA4 Gene ID: 2043 Accession #: P54764 ELISA Kit: RayBio Human Phosphotyrosine EphA4 ELISA	EphA5 Gene Symbol: EphA5 Gene ID: 2044 Accession #: P54756 ELISA Kit: RayBio Human Phosphotyrosine EphA5 ELISA
EphA6 Gene Symbol: EphA6 Gene ID: 285220 Accession #: Q9UF33 ELISA Kit: RayBio Human Phosphotyrosine EphA6 ELISA	EphA7 Gene Symbol: EphA7 Gene ID: 2045 Accession #: Q15375 ELISA Kit: RayBio Human Phosphotyrosine EphA7 ELISA	EphA8 Gene Symbol: EphA8 Gene ID: 2046 Accession #: P29322 ELISA Kit: RayBio Human Phosphotyrosine EphA8 ELISA	EphB1 Gene Symbol: EphB1 Gene ID: 2047 Accession #: P54762 ELISA Kit: RayBio Human Phosphotyrosine EphB1 ELISA	EphB2 Gene Symbol: EphB2 Gene ID: 2048 Accession #: P29323 ELISA Kit: RayBio Human Phosphotyrosine EphB2 ELISA
EphB3 Gene Symbol: EphB3 Gene ID: 2049 Accession #: P54753 ELISA Kit: RayBio Human Phosphotyrosine EphB3 ELISA	EphB4 Gene Symbol: EphB4 Gene ID: 2050 Accession #: P54760 ELISA Kit: RayBio Human Phosphotyrosine EphB4 ELISA	EphB6 Gene Symbol: EphB6 Gene ID: 2051 Accession #: O15197 ELISA Kit: RayBio Human Phosphotyrosine EphB6 ELISA	ErbB2 Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626 ELISA Kit: RayBio Human Phosphotyrosine Erb-B2 ELISA	ErbB3 Gene Symbol: ErbB3 Gene ID: 2065 Accession #: P21860 ELISA Kit: RayBio Human Phosphotyrosine Erb-B3 ELISA
ErbB4 Gene Symbol: ErbB4 Gene ID: 2066 Accession #: Q15303 ELISA Kit: RayBio Human Phosphotyrosine Erb-B4 ELISA	FAK Gene Symbol: PTK2 Gene ID: 5747 Accession #: Q05397 ELISA Kit: RayBio Human Phosphotyrosine FAK ELISA	FER Gene Symbol: FER Gene ID: 2241 Accession #: P16591 ELISA Kit: RayBio Human Phosphotyrosine FER ELISA	FGFR1 Gene Symbol: FGFR1 Gene ID: 2260 Accession #: P11362 ELISA Kit: RayBio Human Phosphotyrosine FGFR1 ELISA	FGFR2 Gene Symbol: FGFR2 Gene ID: 2263 Accession #: P21802 ELISA Kit: RayBio Human Phosphotyrosine FGFR2 ELISA
FGFR2 alpha isoform Gene Symbol: FGFR2 Gene ID: 2263 Accession #: P21802	Fgr Gene Symbol: FGR Gene ID: 2268 Accession #: P09769 ELISA Kit: RayBio Human Phosphotyrosine FGR ELISA	FRK Gene Symbol: FRK Gene ID: 2444 Accession #: P42685 ELISA Kit: RayBio Human Phosphotyrosine FRK ELISA	Fyn Gene Symbol: FYN Gene ID: 2534 Accession #: P06241 ELISA Kit: RayBio Human Phosphotyrosine FYN ELISA	Hck Gene Symbol: HCK Gene ID: 3055 Accession #: P08631 ELISA Kit: RayBio Human Phosphotyrosine HCK ELISA
HGFR Gene Symbol: MET Gene ID: 4233 Accession #: P08581 ELISA Kit: RayBio Human HGF R ELISA	IGF-1 R Gene Symbol: IGF1R Gene ID: 3480 Accession #: P08069 ELISA Kit: RayBio Human Phosphotyrosine IGF-1R ELISA	Insulin R(CD220) Gene Symbol: INSR Gene ID: 3643 Accession #: P06213 ELISA Kit: RayBio Human Phosphotyrosine Insulin R ELISA	Itk Gene Symbol: ITK Gene ID: 3702 Accession #: Q08881 ELISA Kit: RayBio Human Phosphotyrosine ITK ELISA	JAK1 Gene Symbol: JAK1 Gene ID: 3716 Accession #: P23458 ELISA Kit: RayBio Human Phosphotyrosine JAK1 ELISA
JAK2 Gene Symbol: JAK2 Gene ID: 3717 Accession #: O60674 ELISA Kit: RayBio Human Phosphotyrosine JAK2 ELISA	JAK3 Gene Symbol: JAK3 Gene ID: 3718 Accession #: P52333 ELISA Kit: RayBio Human Phosphotyrosine JAK3 ELISA	LCK Gene Symbol: LCK Gene ID: 3932 Accession #: P06239 ELISA Kit: RayBio Human Phosphotyrosine LCK ELISA	LTK Gene Symbol: LTK Gene ID: 4058 Accession #: P29376 ELISA Kit: RayBio Human Phosphotyrosine LTK ELISA	Lyn Gene Symbol: LYN Gene ID: 4067 Accession #: P07948 ELISA Kit: RayBio Human Phosphotyrosine LYN ELISA
MATK Gene Symbol: MATK Gene ID: 4145 Accession #: P42679 ELISA Kit: RayBio Human Phosphotyrosine MATK ELISA	M-CSF R Gene Symbol: CSF1R Gene ID: 1436 Accession #: P07333 ELISA Kit: RayBio Human Phosphotyrosine MCSFR ELISA	MUSK Gene Symbol: MuSK Gene ID: 4593 Accession #: O15146 ELISA Kit: RayBio Human Phosphotyrosine MUSK ELISA	NGFR(TNFRSF16) Gene Symbol: NGFR Gene ID: 4804 Accession #: P08138 ELISA Kit: RayBio Human Phosphotyrosine NGFR ELISA	PDGFRA Gene Symbol: PDGFRA Gene ID: 5156 Accession #: P16234 ELISA Kit: RayBio Human Phosphotyrosine PDGFRA ELISA
PDGFRB Gene Symbol: PDGFRB Gene ID: 5159 Accession #: P09619 ELISA Kit: RayBio Human Phosphotyrosine PDGFRB ELISA	PYK2 Gene Symbol: PTK2B Gene ID: 2185 Accession #: Q14289 ELISA Kit: RayBio Human Phosphotyrosine PYK2 ELISA	RET Gene Symbol: RET Gene ID: 5979 Accession #: P07949 ELISA Kit: RayBio Human Phosphotyrosine RET ELISA	ROR1 Gene Symbol: ROR1 Gene ID: 4919 Accession #: Q01973 ELISA Kit: RayBio Human Phosphotyrosine ROR1 ELISA	ROR2 Gene Symbol: ROR2 Gene ID: 4920 Accession #: Q01974 ELISA Kit: RayBio Human Phosphotyrosine ROR2 ELISA
ROS Gene Symbol: ROS1 Gene ID: 6098 Accession #: P08922 ELISA Kit: RayBio Human Phosphotyrosine ROS ELISA	RYK Gene Symbol: RYK Gene ID: 6259 Accession #: P34925 ELISA Kit: RayBio Human Phosphotyrosine RYK ELISA	SCF R(CD117/c-kit) Gene Symbol: KIT Gene ID: 3815 Accession #: P10721 ELISA Kit: RayBio Human Phosphotyrosine SCFR ELISA	SRMS Gene Symbol: SRMS Gene ID: 6725 Accession #: Q9H3Y6 ELISA Kit: RayBio Human Phosphotyrosine SRMS ELISA	SYK Gene Symbol: SYK Gene ID: 6850 Accession #: P43405 ELISA Kit: RayBio Human Phosphotyrosine SYK ELISA
Tec Gene Symbol: TEC Gene ID: 7006 Accession #: P42680 ELISA Kit: RayBio Human Phosphotyrosine TEC ELISA	Tie-1 Gene Symbol: TIE1 Gene ID: 7075 Accession #: P35590 ELISA Kit: RayBio Human Phosphotyrosine TIE1 ELISA	Tie-2 Gene Symbol: TEK Gene ID: 7010 Accession #: Q02763 ELISA Kit: RayBio Human Phosphotyrosine TIE2 ELISA	TNK1 Gene Symbol: TNK1 Gene ID: 8711 Accession #: Q13470 ELISA Kit: RayBio Human Phosphotyrosine TNK1 ELISA	TRKB Gene Symbol: NTRK2 Gene ID: 4915 Accession #: Q16620 ELISA Kit: RayBio Human Phosphotyrosine TRKB ELISA
TXK Gene Symbol: TXK Gene ID: 7294 Accession #: P42681 ELISA Kit: RayBio Human Phosphotyrosine TXK ELISA	Tyk2 Gene Symbol: TYK2 Gene ID: 7297 Accession #: P29597 ELISA Kit: RayBio Human Phosphotyrosine TYK2 ELISA	TYRO10(DDR2/TKT) Gene Symbol: DDR2 Gene ID: 4921 Accession #: Q16832 ELISA Kit: RayBio Human Phosphotyrosine TYRO10 ELISA	VEGFR2 Gene Symbol: KDR Gene ID: 3791 Accession #: P35968 ELISA Kit: RayBio Human VEGF R2 ELISA	VEGFR3 Gene Symbol: FLT4 Gene ID: 2324 Accession #: P35916 ELISA Kit: RayBio Human Phosphotyrosine VEGFR3 ELISA
ZAP70 Gene Symbol: ZAP70 Gene ID: 7535 Accession #: P43403 ELISA Kit: RayBio Human Phosphotyrosine ZAP70 ELISA

Species Detected

Human

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human RTK Phosphorylation Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Anti-Phospho Tyrosine Detection Antibody
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer (1 x 5 ml)
Protease Inhibitor Cocktail
Phosphatase Inhibitor Cocktail II
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE"s ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Long, Zhi H., et al. "miR-141 inhibits proliferation and migration of colorectal cancer SW480 cells."?Anticancer research?37.8 (2017): 4345-4352.Species: HumanSample type: Cell Lysates
Wang Z., Gao Q., Shi J., et al. Protein tyrosine phosphatase receptor S acts as a metastatic suppressor in hepatocellular carcinoma by control of epithermal growth factor receptor-induced epithelial-mesenchymal transition. Hepatology. 2015 Oct;62(4):1201-14. doi: 10.1002/hep.27911. Species: HumanSample type: Cell Lysate
Cortelazzi B., Verderio P., Ciniselli CM., et al. Receptor tyrosine kinase profiles and human papillomavirus status in oropharyngeal squamous cell carcinoma.J Oral Pathol Med. 2014 Dec 14. doi: 10.1111/jop.12301. Epub ahead of printSpecies: HumanSample type: Tissue Lysate
Acquired resistance to sunitinib in human renal cell carcinoma cells is mediated by constitutive activation of signal transduction pathways associated with tumour cell proliferationSpecies: HumanSample type: Conditioned Media
Al-aidaroos A., et al. Metastasis-associated PRL-3 induces EGFR activation and addiction in cancer cells. J Clin Invest. 2013;123(8):3459-3471. doi:10.1172/JCI66824. Species: HumanSample type: Cell Lysate
Siu IM., et al. Erlotinib Inhibits Growth of a Patient-Derived Chordoma Xenograft . Published: November 15, 2013 DOI: 10.1371/journal.pone.0078895Species: HumanSample type: Cell Lysate
Guerra-Laso, JosÃ? M., et al. "Macrophages from elders are more permissive to intracellular multiplication of Mycobacterium tuberculosis."?Age?35.4 (2013): 1235-1250. Species: HumanSample type: Cell Lysate
Tirado-GonzÃ?lez, I., et al. "Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy."?Molecular human reproduction?19.1 (2013): 43.Species: HumanSample type: Cell Lysate
Strevens, Helena, et al. "PP038. Renal ETK/BMX activation decreased in preeclampsia."?Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health?3.2 (2013): 80.Species: HumanSample type: Tissue Lysate
Kato K., Gong J., Iwama H., et al. The antidiabetic drug metformin inhibits gastric cancer cell proliferation in vitro and in vivo. Mol Cancer Ther. 2012 Mar;11(3):549-60. doi: 10.1158/1535-7163.MCT-11-0594Species: HumanSample type: Cell Lysate

Kit worked pretty well. It would be nice if the rows and columns were labelled somehow. It was somewhat difficult to to determine which row was which if two rows next to each other had no RTKs with signal. Protocol was easy to follow.

Matt SwiatnickiMichigan State University11/13/2019, 02:47 PM

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