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Moraxella bovis
CMX Buffer 1
One unit is the amount of enzyme required to completely digest 1 µg of unmethylated lambda DNA in 1 hour in a total reaction volume of 50 µl
Yes
37°C
10 minutes at 65°C
unmethylated lambda DNA
CMX Buffer 1:
10 mM Tris-HCl (pH 7.0 at 37°C)
10 mM MgCl2
1 mM DTT
Note: 100 ug/ml bovine serum albumin is included in the assay but added separately from the reaction buffer
Buffer 1: 100%
Buffer 2: 50%
Buffer 3: 25%
Buffer 4: 75%
Buffer 5: 75%
*Star activity
10 mM Tris-HCl (pH 7.5 at 4°C)
0.1 mM EDTA
50 mM KCl
1 mM dithiothreitol
0.01% Triton X-100™
100 ug/ml bovine serum albumin
50% glycerol
50 mM Tris‑HCl (pH 7.0 at 37°C)
10 mM MgCl2
100 mM NaCl
1 mM DTT
100 µg/ml BSA
1 µg unmethylated lambda DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl
Store at -20°C
Shipped on dry ice
Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot
Restriction Endonucleases
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn