- Specifications
- Tech Info / Data
- Citations
- Documentation
Specifications
| Formulation | Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA) |
| Antigen | Human interferon beta |
| Isotype | IgG1 |
| Host Species | Mouse |
| Purity | > 95% by SDS-PAGE stained by Coomassie BlueEndotoxin level < 1 EU/μg |
| Specificity | Binds to human IFN-beta with high affinity |
| Storage | For retention of full activity store at -20°C or below and avoid repeated freeze/thaw cycles |
| Antigen Synonyms | IFN Beta |
| Clone | MMHB-12 |
Tech Info / Data
Tested Application:
- Direct Binding ELISA
Citations
Documentation
21450-1 Certificate of Analysis (CoA) SDSRepresentative Binding Curve
Representative binding curve of antibody to mammalian expressed human interferon beta 1a (circle) and to 0.1% BSA/PBS (square) in a Direct Binding ELISA. Medium-binding polystyrene plates were coated with either 1 µg/ml human interferon beta 1a or 0.1% BSA/PBS. Thereafter, titrations of the product were added to the wells. Donkey anti-mouse IgG conjugated to HRP was used as the detection antibody. Colorimetric detection was performed using 3,3",5,5"-Tetramethylbenzidine (TMB) substrate. The HRP-TMB reaction was stopped using a H2SO4/HCl solution.