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产品资料

BD Biosciences/FITC Rat Anti-Mouse IgG3/0.5mg/553403

Product DetailsRecommended AssayReferences

Description

The R40-82 antibody specifically recognizes mouse IgG3 of all strains. It does not react with other Ig isotypes.

Format

FormatFITC
Excitation SourceBlue 488 nm
Excitation Max494nm
Emission Max520nm

FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.

Other Formats→

BB700
BUV395
BUV496
BUV615

BUV661
BV421
BV480
BV510

BV605
BV650
BV711
BV750

BV786
Biotin

Suggested Companion Products

FITC Rat IgG2a, κ Isotype Control RUO 0.1mgCat No: 554688

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) 2.4G2RUO 0.1mgCat No: 553141

BD Cytofix/Cytoperm™ Fixation/Permeablization Kit RUO 250TestsCat No: 554714

Stain Buffer (FBS) RUO 500mLCat No: 554656

Stain Buffer (BSA) RUO 500mLCat No: 554657

Resources & Tools
Spectrum Viewer	Panel Designer	SpectrumViewer	Download MSDS	Download TDS

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light.Do not freeze.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

FITC Rat Anti-Mouse IgG3 may be used as a primary or secondary reagent in immunofluorescent staining. FITC Rat Anti-Mouse IgG3 is effective for detection of cell-surface or intracellular Ig by immunofluorescent staining with flow cytometric analysis. Note: intracellular staining is not routinely tested at BD Biosciences Pharmingen. The BD Cytofix/Cytoperm™ Fixation/Permeablization Kit, (Cat. No. 554714) contains the appropriate reagents for intracellular staining.

Duong BH, Ota T, Aït-Azzouzene D, et al. Peripheral B cell tolerance and function in transgenic mice expressing an IgD superantigen.. J Immunol. 2010; 184(8):4143-58. (Biology: Flow cytometry, Intracellular staining (flow cytometry)).View reference
Kuzin II, Snyder JE, Ugine GD, et al. Tetracyclines inhibit activated B cell function.. Int Immunol. 2001; 13(7):921-31. (Biology: Flow cytometry, Intracellular staining (flow cytometry)).View reference