UsetheCFX384TouchReal-TimePCRDetectionSystemforprecise,reproducIBLereal-timePCR.ThesystemiscomposedoftheCFX384OpticalReactionModuleandtheC1000TouchThermalCyclerChassis.ThemoduleisamemberofthefamilyofinterchangeablereactionmodulesthatcanbeusedwiththeC1000TouchThermalCyclerChassisforPCRandreal-timePCR.Allmodulescanbequicklyinsertedandremovedfromthecyclerchassiswithouttools.
TheCFX384TouchReal-TimePCRDetectionSystemhassixindependentlycontrolledthermalelectricunitsprovidingeven,preciselycontrolledtemperaturesatalltimesduringtherun,includingramping.Thereducedmassofthehoneycombblockprovidesfastrampingandreducedsettlingtime(timetoachievethermaluniformity).
Theopticalsystem,withfivefilteredLEDsandfivefilteredphotodiodes,collectsdatafromallwells,detectinguptofourtargetsperwell.Forsingle-colorFAMandSYBR®GreenI,thefastscanoptionreadssingle-channelfluorescenceinall384wellsinjust8seconds.AchannelwithanLEDfilter–photodiodecombinationisdedicatedtoFRETsingleplexexperiments.
Thelargetouch-screeninterfacehasintuitivegraphicalprogramminganddisplaysdatatracesinrealtime.Theprogrammablethermalgradientfeaturecantesteighttemperaturesatoncetoquicklydeterminetheoptimalannealingtemperature,andtheprotocolautowriterquicklyoptimizesreactions,generatinganoptimalprotocolbasedonyourpolymerase,primers,andproductlength.
ThefollowingmodulescanbeusedwiththeC1000TouchThermalCyclerChassis:
ReactionModules | CatalogNumber | |
Module | ModulewithStarterPackage* | |
ForPCR | ||
Dual48/48FastReactionModule | 1840148 | — |
96-WellFastReactonModule | 1840196 | — |
96–DeepWellReactionModule | 1840197 | — |
384-WellReactionModule | 1840138 | — |
ForReal-TimePCR | ||
CFX96™OpticalReactionModule | 1845097 | 1845096 |
CFX96DeepWellOpticalReactionModule | 1844095 | 1844096 |
CFX384OpticalReactionModule | 1845385 | 1845384 |
TheCFX384TouchReal-TimePCRDetectionSystemcanbeconfiguredwithotherPCRinstruments,allowingstand-aloneorsoftwarecontrol.InstrumentpermutationsallowuptofourdifferentCFXConnect™,CFX96,orCFX384SystemstobecontrolledbyonePC.
CombineanytwoCFXReal-TimePCRDetectionSystemswiththeCFXAutomationSystemII,ourroboticplatehandler,tocreateahigh-throughput,automatedreal-timePCRsystemwithacompactfootprint.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn