Technical Details
Human IL-21, accession # Q9HBE4, Gln25-Ser155
Lyophilized from 200 µl of a 0.2 µm-filtered solution containing 15 mM sodium acetate and 45 mM sodium chloride, pH 5.5
Both product grades are produced under the same conditions in a GMP facility, ensuring an equal product quality and performance. We offer a more comprehensive QC testing including tighter specifications and documentation for our GMP products: Preclinical vs GMP.
| Preclinical | GMP | |
| Molecular weight | 15.4 kDa | 15.4 kDa |
| Purity | ≥ 95% as determined by SDS-PAGE | ≥ 97% as determined by SDS-PAGE |
| Activity | ≥ 0.5 x 106 U/mg, calibrated against internal standard “IL-21 RS1” Measured in a cell proliferation assay using an IL-21-dependent cell line, mouse hybridoma B-cells (B9) | 0.5 – 1.6 x 106 U/mg, calibrated against internal standard “IL-21 RS1” Measured in a cell proliferation assay using an IL-21-dependent cell line, mouse hybridoma B-cells (B9) Batch specific activity on CoA |
| Endotoxin level | < 25 EU/mg | ≤ 25 EU/mg |
| Intended use | Intended for preclinical ex vivo use. Not intended for therapeutic use. | Intended for clinical ex vivo use. Not intended for human in vivo application. |
Handling Instructions
Recommended in sterile water to a final concentration of 250 µg/ml.
Ambient temperature. Please refer to Technote to learn more about our shipment validation procedure.
≥ 6 months from date of shipping. Please refer to Certificate of Analysis for the exact expiry date.
Store lyophilized cytokine at -20°C to -80°C.
Store a 100 µg/ml reconstituted cytokine solution:
• 4 weeks at 2°C to 8°C under sterile conditions after reconstitution. Store in the original container.
• 4 months at -20°C to -80°C under sterile conditions after reconstitution. Store in 60 µl aliquots in polypropylene cryogenic vials.
Avoid repeated freeze/thaw cycles.
