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| Product Code | opr0009 |
|---|---|
| Constituents | Reagent Cov A Reagent Cov B |
| Lysat Type | "0 |
| Principle | Chemiluminescence is defined as the light emitted by a chemical reaction. Horse radish peroxidase (HRP), in the presence of hydrogen peroxide, catalyses the oxidation of a cyclic diacyl hydrazide, such as luminol, to an excited reaction product which on spontaneous change to a stable form emits light. A dramatic increase in the light output can be achieved by the presence of enhancers. |
|---|---|
| Applications | Western Blot, Dot Blot |
| Recommendations | Chemiluminescence is defined as the light emitted by a chemical reaction. Horse radish peroxydase (HRP), in the presence of hydrogen peroxide, catalyses the oxidation of a cyclic diacyl hydrazide, such as luminol, to an excited reaction product which on s |
| Storage | Stored at +4°C |
| Product type | Reagents |
| Preparation method | To be used extemporaneously or can be kept at +4°C for few hours : Add 1 drop of oxidant Cov B to 1 mL of substrat Cov A (125 µL of reagent covers 1 cm2 membrane). Detection : Immerse the membrane in Covalight reagent from 1 to 10 minutes. Shake of excess reagent and cover the membrane with a plastic sheet before to exposure. |
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