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5339:Recombinant Rat Interleukin-4 PRODUCT DESCRIPTION: Recombinant Rat IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 126 amino acids and having a molecular mass of 14105 Dalton. The rrIL-4 is purified by proprietary chromatographic techniques. SOURCE: Escherichia Coli. FORMULATION: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. PURITY: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. (c) Anion-exchange FPLC STORAGE: Although stable at room temperature for 3 weeks, it should be stored desiccated below -18°C. Upon reconstitution store at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. SOLUBILITY: It is recommended to reconstitute in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. BIOLOGICAL ACTIVITY: Recombinant Rat IL-4 is fully biologically active when compared to standard. The ED50=1-3 ng/mL. The biological activity is determined by measuring the dose dependent increase in bromodeoxyuridine (BrdU) incorporation in rat splenocytes. A concentration range of 0.1 to 10.0 ng/mL is effective for most in vitro applications. The optimal concentration should be determined for each specific application. |
DevaTal, Inc, 644 Newkirk Ave, Hamilton NJ 08610 Phone: 609-586-1575, Fax: 609-586-6892 |
FOR RESEARCH USE ONLY |
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