Mouse
Multiplex
Flow Cytometry
2 hours
This GeniePlex Genieplex Mouse Inflammation 8-Plex Immunoassay Panel is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes IFN-Gamma, IL-1-Alpha, IL-6, IL-10, IL-12p70, KC, MCP-1, TNF-Alpha on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.
GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.
At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 8 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.
As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.
Reactivity: | Mouse |
Analytes: | IFN-Gamma, IL-1-Alpha, IL-6, IL-10, IL-12p70, KC, MCP-1, TNF-Alpha |
1x Ab-conjugated beads (premixed): | S4P1 - anti-M IL-1-Alpha; S4P4 - anti-M TNF-Alpha; S4P7 - anti-M IL-12p70; S4P8 - anti-M IFN-Gamma; S5P2 - anti-M KC; S5P4 - anti-M MCP-1; S5P5 - anti-M IL-6; S5P9 - anti-M IL-10 |
2x Biotin-detection Ab (premixed): | Biotin - anti-M IL-1-Alpha; Biotin - anti-M TNF-Alpha; Biotin - anti-M IL-12p70; Biotin - anti-M IFN-Gamma ; Biotin - anti-M KC; Biotin - anti-M MCP-1; Biotin - anti-M IL-6; Biotin - anti-M IL-10 |
Buffers: | 2x Mouse/Rat dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent. |
Lyophilized Standard Mix: | Lyophilized Standard Mix-Mouse Inflammation 8-Plex.One vial containing lyophilized recombinant IFN-Gamma, IL-1-Alpha,IL-6, IL-10, IL-12p70, KC, MCP-1 and TNF-Alpha. |
Sample types: | Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate |
Sensitivity (LODs): | IFN-Gamma, < 1="" pg/ml;="" il-1-alpha,="">< 2="">< 3="">< 2="" pg/ml;="" il-12p70,="">< 1="" pg/ml;="" kc,="">< 1="" pg/ml; mcp-1,="">< 1="" pg/ml; tnf-alpha,="">< 0.5=""> |
LLOQs: | IFN-Gamma, < 2="" pg/ml;="" il-1-alpha,="">< 10="">< 10="">< 5="" pg/ml;="" il-12p70,="">< 2="" pg/ml;="" kc,="">< 2="" pg/ml; mcp-1,="">< 2="" pg/ml; tnf-alpha,="">< 2=""> |
ULOQs: | IFN-Gamma, > 5,000 pg/mL; IL-1-Alpha, > 5,000 pg/mL; IL-6, > 5,000 pg/mL; IL-10, > 2,000 pg/mL; IL-12p70, > 2,000 pg/mL; MCP-1, > 2,000 pg/mL; KC, > 2,000 pg/mL; TNF-Alpha, > 1,500 pg/mL.. |
Standard dose recovery: | Standard dose recovery: 70-130% |
Intra-assay CV: | Intra-assay CV: <> |
Inter-assay CV: | Inter-assay CV: <> |
Cross-reactivity of analytes in the Panel: | Cross-reactivity of analytes in the Panel: Negligible |
Sample volume: | Sample volume: 15 µL/test |
Datasheets
Reactivity: | Mouse |
ELISA Type: | Multiplex |
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