Deprecated: Creation of dynamic property cls_session::$session_data_table is deprecated in /www/sites/www.188bio.com/index/systems/cls_session.php on line 49
ELISAGenie/Human AQP-3 (Aquaporin 3) CLIA Kit (HUES00351)/Aquaporin 3) CLIA Kit (HUES00351/HUES00351188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
您好,欢迎您进入188进口试剂采购网网站! 服务热线:4000-520-616
蚂蚁淘商城 | 现货促销 | 科研狗 | 生物在线
产品资料

ELISAGenie/Human AQP-3 (Aquaporin 3) CLIA Kit (HUES00351)/Aquaporin 3) CLIA Kit (HUES00351/HUES00351

  • Description
  • Additional Information

Description

ELISA Kit Technical ManualMSDS

Human AQP-3 (Aquaporin 3) CLIA Kit

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AQP-3 . Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AQP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AQP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AQP-3. The concentration of Human AQP-3 in the samples can be calculated by comparing the RLU of the samples to the standard curve.

Assay typeSandwich
Format96T
Assay time4.5h
ReactivityHuman
Detection methodChemiluminescence
Detection range78.13-5000 pg/mL
Sensitivity46.88 pg/mL
Sample volume100uL
Sample typeSerum, plasma and other biological fluids
RepeatabilityCV < 15%

Specificity

This kit recognizes Human AQP-3in samples. No significant cross-reactivity or interference between Human AQP-3and analogues was observed.

Typical Data

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL) RLU Average Corrected
500055555669576125661233
250033402353063435434331
125018696176421816918146
6258879990593929369
312.54907475948334810
156.252601242125112488
78.131298138013391316
0232323--

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human AQP-3were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human AQP-3were tested on 3 different plates, 20 replicates in each plate.

Intra-assay Precision Inter-assay Precision
Sample123123
n202020202020
Mean (pg/mL)257.30592.152128.75259.71623.531968.13
Standard deviation26.2747.19132.4127.4873.45190.91
C V (%)10.217.976.2210.5811.789.70

Recovery

The recovery of Human AQP-3spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum (n=5)97-113104
EDTA plasma (n=5)94-107101
Cell culture media (n=5)87-9993

Linearity

Samples were spiked with high concentrations of Human AQP-3and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2Range (%)84-9896-10894-109
Average (%)91102100
1:4Range (%)101-11388-10087-104
Average (%)1069395
1:8Range (%)86-9986-9987-99
Average (%)939193
1:16Range (%)100-11595-10992-106
Average (%)106101100

Kit Components & Storage

An unopened kit can be stored at 4"C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item Specifications Storage
Micro CLIA Plate(Dismountable)8 wells X12 strips-20"C, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100X)1 vial, 120 uL
Concentrated HRP Conjugate (100X)1 vial, 120 uL-20"C(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL4"C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25X)1 vial, 30 mL
Substrate Reagent A1 vial, 5 mL4"C (shading light)
Substrate Reagent B1 vial, 5 mL4"C (shading light)
Plate Sealer5 pieces
Product Description1 copy
Certificate of Analysis1 copy

Human AQP-3 (Aquaporin 3) CLIA Kit(HUES00351) Assay procedure

    1. Set standard, test sample and control (zero) wells on the pre-coated plate and record theirpositions. It is recommended to measure each standard and sample in duplicate. Note: addall solutions to the bottom of the plate wells while avoiding contact with the well walls. Ensuresolutions do not foam when adding to the wells.
    2. Aliquot 100µl of standard solutions into the standard wells.
    3. Add 100µl of Sample / Standard dilution buffer into the control (zero) well.
    4. Add 100µl of properly diluted sample (serum, plasma, tissue homogenates and otherbiological fluids.) into test sample wells.
    5. Cover the plate with the sealer provided in the kit and incubate for 90 min at 37°C.
    6. Aspirate the liquid from each well, do not wash. Immediately add 100µL of BiotinylatedDetection Ab working solution to each well. Cover the plate with a plate seal and gently mix.Incubate for 1 hour at 37°C.
    7. Aspirate or decant the solution from the plate and add 350µL of wash buffer to each welland incubate for 1-2 minutes at room temperature. Aspirate the solution from each well andclap the plate on absorbent filter paper to dry. Repeat this process 3 times. Note: a microplatewasher can be used in this step and other wash steps.
    8. Add 100µL of HRP Conjugate working solution to each well. Cover with a plate seal andincubate for 30 min at 37°C.
    9. Aspirate or decant the solution from each well. Repeat the wash process for five times asconducted in step 7.
    10. Add 100µL of Substrate mixture solution to each well. Cover with a new plate seal andincubate for no more than 5 min at 37°C. Protect the plate from light.
    11. Determine the RLU value of each well immediately.

Human AQP-3 (Aquaporin 3) CLIA Kit(HUES00351) Protein Information

UniProt Protein Function:AQP3: Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism. Belongs to the MIP/aquaporin (TC 1.A.8) family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transporter; Channel, misc.; Membrane protein, integral; Membrane protein, multi-pass; Transporter, aquaporin family

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: integral to plasma membrane; basolateral plasma membrane; cytoplasm; integral to membrane; plasma membrane; intercellular junction

Molecular Function:transporter activity; urea transmembrane transporter activity; water channel activity; glycerol channel activity

Biological Process: glycerol transport; positive regulation of immune system process; cellular water homeostasis; response to retinoic acid; water transport; excretion; odontogenesis; response to vitamin D; transport; renal water homeostasis; response to calcium ion; regulation of keratinocyte differentiation; transmembrane transport

Disease: Gil Blood Group

NCBI Summary:This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. [provided by RefSeq, Aug 2011]
UniProt Code:Q92482
NCBI GenInfo Identifier:2497938
NCBI Gene ID:360
NCBI Accession:Q92482.2
UniProt Secondary Accession:Q92482,O00108, Q6FGT2, Q6FGW6, A8K843, B2RE16, D3DRL3
UniProt Related Accession:Q92482
Molecular Weight:29,987 Da
NCBI Full Name:Aquaporin-3
NCBI Synonym Full Names:aquaporin 3 (Gill blood group)
NCBI Official Symbol:AQP3  
NCBI Official Synonym Symbols:GIL; AQP-3  
NCBI Protein Information:aquaporin-3; aquaglyceroporin-3; aquaporin 3 (GIL blood group)
UniProt Protein Name:Aquaporin-3
UniProt Synonym Protein Names:Aquaglyceroporin-3
Protein Family:Aquaporin
UniProt Gene Name:AQP3  
UniProt Entry Name:AQP3_HUMAN
View AllClose

Additional Information

Reactivity:
Human
ELISA Type:
CLIA
View AllClose
新闻动态
行业前沿
技术文章
最新产品