首页/试剂/AmpliCap-Max™T7HighYieldMessageMakerKit
TheAmpliCap-Max™T7HighYieldMessageMakerKitproducescappedRNAbyinvitrotranscriptionusingT7RNApolymeraseandthestandardcapanalogm7G[5"]ppp[5"]G.A30minute,20μlAmpliCapMaxreactionyieldsupto60μgofm7G-cappedRNAfrom1μgofthe1.4kbstandardT7controltemplateDNA.Them7G[5"]ppp[5"]Gcapanalog/NTPPreMixcontainsallfourribonucleotidesandthemonomethylatedcapanalog.ThePreMixensurestheoptimalconcentrationofeachNTPandratioofmethylatedcapanalogtoGTP(4:1),maximizingtranscriptcapping(~80%)andyield.BecausetheconcentrationofGTPinthereactionislimiting,thecapanalogispreferentiallyincorporatedasthefirstor5"-terminalGofthetranscript.CELLSCRIPTalsoofferstheMessageMAX™T7ARCA-CappedMessageTranscriptionKitwhichalsoproducesupto60μgofcappedRNAina30minutestandardcontrolreactionwithan80%cappingefficiency.TheMessageMAXKitusestheanti-reversecapanalog(ARCA),m27,3"-OG[5"]ppp[5"].
品牌名称：
CELLSCRIPT
品牌logo：
品牌介绍：CELLSCRIPT总代理 CELLSCRIPT一级代理 CELLSCRIPT特约代理 CELLSCRIPT全国代理 CELLSCRIPT代理
品牌官网：
http://www.cellscript.com
品牌介绍：
Themissionof CELLSCRIPT™ istoprovidethebestproductsandtechnologiesformakingandusingRNAfortranslationincellsforclinicalresearchandtherapeutics.
Currentproductsincludekitsfor invitro transcription,5"RNAcappingusingeitheracapanalogorcappingenzymes,and3"RNApolyadenylation,aswellasall-in-onekitsformakingcapped,poly(A)-tailedmRNAfortranslationincells.
CELLSCRIPT™ recentlyintroducedINCOGNITO™RNATranscriptionKitsfor invitrosynthesisofRNAthatcontainsmodifiednucleosides,suchaspseudouridine(Ψ)and/or5-methylcytidine(m5C)inplaceofthecorrespondingUorCcanonicalnucleosides(Figure1).Thesekitsareso-namedbecausethecapped,polyadenylatedandnucleoside-modifiedRNAproducts(calledINCOGNITOmRNAs)aredisguisedsotheydonotinduceinnateimmuneresponsestothesameextentasthecorrespondingunmodifiedmRNAswhentransfectedintomammaliancellsthatexpressavarietyofRNAsensors.
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产品相关链接：
1ADPP/N384
2Collagen P/N385
3ThrombinP/N386
4ArachidonicAcidP/N390
5EpinephrineP/N393
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