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ATTO647-Actin for TIRFMα-actin from skeletal muscle, rabbitCat. #: 8158-04 .............................................................................................................................................................................................................................................................................................................................................................................................................
Description
| |
Protein | ATTO647-Actin |
Origin | Skeletal muscle alpha actin, rabbit |
Molecular mass | 42kDa |
Protein description | Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. ATTO647-Actin is chemically modified G-actin with an ATTO647 fluorescent group covalently bound to lysine residues.ATTO647-Actin(λex647nm,λem669nm)possesses the polymerization properties of native G-actin. |
Conjugation ratio | This product is supplied with a degree of labelling (DOL) of ~ 0.3, suited for TIRF microscopy. |
Properties
| |
Form | Lyophilized, ready-to-use. |
Quantity per unit | 5x100 µg |
Buffer | 2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3and 0.3% disaccharides, when reconstituted with100µl ultrapure H2Oto obtain a 1mg/ml solution. |
Purity | >99%by scanning densitometry. |
Purification notes | Purified from rabbit skeletal muscle. |
Protein concentration | Determined by the Biuret method. |
Storage instructions | ATTO647-Actin stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized productis kept on ice, protect from light and it should be used within 1 week.Avoid refreezing of actin. |
Shipping conditions | At ambient temperature. Upon delivery store at -70°C. |
Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. | |
Further Information
Product DataSheet
Material and Safety Data Sheet
Table of Dyes and Light Sources
Protein Sequence on NCBI
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