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hypermol/Pyrene Actin 100% (rabbit skeletal muscle alpha actin) - 1.0 mg/1x1 mg/8112-01

Description

Pyrene Actin (100%)

α-actin from rabbit skeletal muscleCat. #: 8112-01..........................................................................................................................................................................................................................................................................................................................................................................................................................................................

ApplicationsFluorimetrical Analysis of actin dynamics (e.g. kinetics of polymerization, nucleation, depolymerization)

Description

Pyrene Actin

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Protein description

G-actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. Pyrene-Actinis chemically modified G-actin, carrying a pyrenyl group covalently bound to Cys374 at the C-term of the molecule.The actin polymerization can be inducedsimply by mono- or divalent cations(in-vitro conditions) andthe actin kinetic can then be measuredfluorometrically. Fluorometry with Pyrenyl-Actin is standard method to study the effect of ligands on actin kinetics.

Conjugation ratio

Pyrene Actin (100%) is 100% labelled and thus has a dye/protein molar ratio of 1.

Properties

Form

Lyophilized, ready-to-use.

Quantityper unit

1x1 mg

Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3and 0.3% disaccharides, when reconstituted with 1.0 mlultrapure water to obtain a 1mg/ml solution.

Purity

>99%by scanning densitometry.

Purification notes

Purified by GPC from rabbit skeletal muscle.

Protein concentration

Determined by the Biuret method.

Storage instructions

Pyrene actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized pyrene actin is kept on ice and should be used within 1 week.Avoid refreezing.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

CAS no.

Further Information

Product DataSheetMaterial and Safety Data SheetProtein Sequence on NCBI

References

Cysteine-richprotein2acceleratesactinfilamentcluster formation.Kihara T, Sugimoto Y, Shinohara S, Takaoka S, Miyake J.; PLoS One. 2017 Aug 16;12(8):e0183085. doi: 10.1371/journal.pone.0183085. eCollection 2017.​

Cooperativeeffectsoftropomyosinon thedynamicsof theactin filament.Khaitlina S, Tsaplina O, Hinssen H.; FEBS Lett. 2017 Jul;591(13):1884-1891. doi: 10.1002/1873-3468.12700. Epub 2017 Jun 11.

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