Pyrene Actin (100%)
α-actin from rabbit skeletal muscleCat. #: 8112-01..........................................................................................................................................................................................................................................................................................................................................................................................................................................................
ApplicationsFluorimetrical Analysis of actin dynamics (e.g. kinetics of polymerization, nucleation, depolymerization)
Description
Pyrene Actin | |
Origin | Skeletal muscle alpha actin, rabbit |
Molecular mass | 42kDa |
Protein description | G-actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. Pyrene-Actinis chemically modified G-actin, carrying a pyrenyl group covalently bound to Cys374 at the C-term of the molecule.The actin polymerization can be inducedsimply by mono- or divalent cations(in-vitro conditions) andthe actin kinetic can then be measuredfluorometrically. Fluorometry with Pyrenyl-Actin is standard method to study the effect of ligands on actin kinetics. |
Conjugation ratio | Pyrene Actin (100%) is 100% labelled and thus has a dye/protein molar ratio of 1. |
Properties
Form | Lyophilized, ready-to-use. |
Quantityper unit | 1x1 mg |
Buffer | 2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3and 0.3% disaccharides, when reconstituted with 1.0 mlultrapure water to obtain a 1mg/ml solution. |
Purity | >99%by scanning densitometry. |
Purification notes | Purified by GPC from rabbit skeletal muscle. |
Protein concentration | Determined by the Biuret method. |
Storage instructions | Pyrene actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized pyrene actin is kept on ice and should be used within 1 week.Avoid refreezing. |
Shipping conditions | At ambient temperature. Upon delivery store at -70°C. |
Remarks | |
CAS no. |
Further Information
Product DataSheetMaterial and Safety Data SheetProtein Sequence on NCBI
ReferencesCysteine-richprotein2acceleratesactinfilamentcluster formation.Kihara T, Sugimoto Y, Shinohara S, Takaoka S, Miyake J.; PLoS One. 2017 Aug 16;12(8):e0183085. doi: 10.1371/journal.pone.0183085. eCollection 2017.
Cooperativeeffectsoftropomyosinon thedynamicsof theactin filament.Khaitlina S, Tsaplina O, Hinssen H.; FEBS Lett. 2017 Jul;591(13):1884-1891. doi: 10.1002/1873-3468.12700. Epub 2017 Jun 11.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn