Cap Z (capping protein)(Actin Binding Protein)Cat. #: 8322-01
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Description
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Protein | Cap Z, Capping protein (non-muscle isoform) |
Origin | recombinant, human recombinant |
Molecular mass | 66kDa |
Protein description | The subunits of the heterodimeric capping protein CapZ possess a molecular mass of 34kDa (alpha 1-subunit) and 32kDa (beta 2-subunit, His-tagged). CapZ binds with high affinity in a Ca++-independent manner to the barbed end of actin filaments (1:1000 M/M). |
Actin interaction | Ca++-insensitive barbed end capping protein. Kd actin binding = >1nM (barbed end) . |
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Form | Lyophilized, ready-to-use. |
Quantity per unit | 2x50µg |
Buffer | 150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted with 50 µl ultrapure water to obtain a 1.0 mg/ml solution. |
Purity &Activity | Purity: >94%by scanning densitometryfrom Coomassie G-250 stained SDS-Gels. Capping activity: F-actin (Cat.#:8101) containing 20% pyrene actin (Cat.#: 8112) was incubated with CapZ and diluted below the critical concentration. Depolymerisation was followed by fluorometry and compared to an actin reference without CapZ.Complete capping was obtained at a molar ratio of 1 : 1000 (CapZ : Actin). All proteins were used as supplied, i.e. without dialysis. G-Actin stock solutions were pre-spun 1h, 100.000xg, 4°C. |
Purification notes | The alpha-1 SU is untagged, while the beta-2 SU contains a His-tag. Using anti-His antibodies, the protein can be identified by all suited immunological techniques. |
Protein concentration | Determinedby OD280 (0.1%=1.234). |
Storage instructions | CapZ isstored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilizedproteinis kept on ice and should be stored at -20°C in glycerol orCryoProtect(Cat.#: 6011-01).Avoid repeated freeze / thaw cycles. |
Shipping conditions | At ambient temperature. Upon delivery store at -70°C. |
Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. | |
Further InformationProduct DataSheet
Material and Safety Data SheetSequence on NCBI (alpha-1 SU)Sequence on NCBI (beta-2 SU)
Structure on NCBI (PDB ID: 1IZN)
References
Myosin-II activity generates a dynamic steady state with continuous actin turnover in a minimal actin cortexSonal, Kristina A. Ganzinger, Sven K. Vogel, Jonas Mücksch, Philipp Blumhardt and Petra SchwillebioRxiv preprint first posted online May. 2, 2018; doi: https://dx.doi.org/10.1101/312512
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