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hypermol/Talin (smooth muscle, turkey) - 100µg/8314-01

Description

Talin(Actin Binding Protein)Cat. #: 8314-01

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Description

Protein

Talin (Talin-1)

Origin

Smooth muscle, turkey

Molecular mass

~270kDa

Protein description

Talin isolated from smooth muscle has a molecular mass of ~270kD. Talin consists of a 220kD rod containing actin, vinculin and integrin binding sites and an N-terminal 47kD head region with a FERM domain. The actin-binding multiligand protein Talin interacts e.g. with phospholipids, signaling molecules, and cytoskeletal components.

Actin interaction

Actin binding protein.

Properties

Form

Lyophilized, ready-to-use.

Quantity per unit

100 µg

Buffer

0.1M KCl, 10mM imidazole pH 7.4, 2mM MgCl2, 0.2mM DTT and 2% disaccharides, when reconstituted with 100µlultrapure water to obtain a 1mg/ml solution.

Purity

~94%by scanning densitometryfrom Coomassie G-250 stained SDS-Gels.

Purification notes

Purified from turkey gizzard muscle.

Protein concentration

Determinedby Biuret method.

Storage instructions

Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

Further Information

Product DataSheet
Material and Safety Data SheetProtein Sequence on NCBI

Literature

A high molecular mass protein isolated from chicken gizzard: its localization at the dense plaques and dense bodies of smooth muscle and the Z-disks of skeletal muscle.

Terasaki AG, Nakagawa H, Kotani E, Mori H, Ohashi K.J Cell Sci. 1995 Mar;108 ( Pt 3):857-68

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