Talin(Actin Binding Protein)Cat. #: 8314-01
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Description
Protein | Talin (Talin-1) |
Origin | Smooth muscle, turkey |
Molecular mass | ~270kDa |
Protein description | Talin isolated from smooth muscle has a molecular mass of ~270kD. Talin consists of a 220kD rod containing actin, vinculin and integrin binding sites and an N-terminal 47kD head region with a FERM domain. The actin-binding multiligand protein Talin interacts e.g. with phospholipids, signaling molecules, and cytoskeletal components. |
Actin interaction | Actin binding protein. |
Properties
Form | Lyophilized, ready-to-use. |
Quantity per unit | 100 µg |
Buffer | 0.1M KCl, 10mM imidazole pH 7.4, 2mM MgCl2, 0.2mM DTT and 2% disaccharides, when reconstituted with 100µlultrapure water to obtain a 1mg/ml solution. |
Purity | ~94%by scanning densitometryfrom Coomassie G-250 stained SDS-Gels. |
Purification notes | Purified from turkey gizzard muscle. |
Protein concentration | Determinedby Biuret method. |
Storage instructions | Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles. |
Shipping conditions | At ambient temperature. Upon delivery store at -70°C. |
Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
Further Information
Product DataSheetLiterature
A high molecular mass protein isolated from chicken gizzard: its localization at the dense plaques and dense bodies of smooth muscle and the Z-disks of skeletal muscle.
Terasaki AG, Nakagawa H, Kotani E, Mori H, Ohashi K.J Cell Sci. 1995 Mar;108 ( Pt 3):857-68
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