Myosin II(Actin Binding Protein)Cat. #: 8326-01
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Description
Protein | Myosin II |
Origin | Skeletal muscle, rabbit (m.psoas) |
Molecular mass | ~480kDa |
Protein description | Fast muscle myosin II from rabbit psoas muscle is a hexameric, actin-binding ATPase with a total molecular weight of 480kD in a head- and-tail conformation. Myosin II has two identical head domains, where the actin binding sites are localized. It consists of two heavy chains (each 220kD) and four light chains (ELC=essential and RLC= regulatory light chains). |
Actin interaction | Binds to F-actin. |
Properties
Form | Lyophilized, ready-to-use. |
Quantityper unit | 2x100 µg |
Buffer | 500mM KCl, 20mM Imidazole pH 7.0, 2mM DTT and 8% disaccharides, when reconstituted with 0.1mlultrapure water to obtain a 1mg/ml solution. |
Purity | >98%by scanning densitometryfrom Coomassie G-250 stained SDS-Gels. |
Purification notes | Purified from rabbit skeletal muscle (m. psoas). |
Protein concentration | Determined by Biuret method with actin (rabbit skeletal muscle) as reference. |
Storage instructions | Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles. |
Shipping conditions | At ambient temperature. Upon delivery store at -70°C. |
Remarks | For coated or filamentous myosin II MotorMix I (Cat. #: 5502-01) or PolyMix (Cat. #: 5000-0*) can be used.MotorMix II (Cat. #: 5503-01) is required for soluble myosin II. |
CAS no. |
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