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hypermol/Fascin (human, recombinant) - 2x50 µg/8411-01

Description

Fascin (Fascin 1)(Actin Binding Protein)Cat. #: 8411-01

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Description

Protein

Fascin (Synonyms: Fascin 1,55 kDa actin-bundling protein,Singed-like protein,p55)

Origin

human recombinant - untagged.

Molecular mass

55kDa

Protein description

Fascin is a monomeric actin bundling protein witha molecular mass of 55kDa.

Actin interaction

Ca++-insensitive actin bundling protein.

Properties

Form

Lyophilized, ready-to-use.

Quantity per unit

2x50µg

Buffer

150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted with 50 µlultrapure water to obtain a 1.0 mg/ml solution.

Purity &Activity

Purity: >99%by scanning densitometryfrom Coomassie G-250 stained SDS-Gels.

Bundling activity: 2µMAtto565-Actin(Cat.#:8162-01) was incubated with Fascin at a molar ratio of 1:10 and 1:4.1 to verify bundling by fluorecence microscopy. All proteins were used as supplied, i.e. without dialysis. The actin stock solution was pre-spun 15min, 15.000xg, 4°C.

Low speed cosedimentation assay was performed (20,000xg, 30min) with rabbit skeletal muscleactin(Cat.#:8101-03)in the presence of sugars. At a molar ratio of 4. : 1 (actin : fascin), fascin was mixed with F-actin. After 30min at RT, the samples were used for low speed spinning. SDS-PAGE showed, that fascin was completely pelleted with F-actin.

Purification notes

Fascinprotein was amplified from human macrophage cDNA and expressed as a GST-tag fusion protein in E. coli. After purification by GST-Agarose and subsequent GST-tag cleavage, the pure protein was yielded by gel filtration chromatography.

Protein concentration

Determinedby OD280 (0.1%=1.224).

Storage instructions

Fascin isstored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilizedproteinis kept on ice and should be stored at -20°C in glycerol orCryoProtect(Cat.#: 6011-01).Avoid repeated freeze / thaw cycles.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

CAS no.

Further Information

Product DataSheetProtein Sequence on NCBIProtein Sequences and Structures on RCSB
Material and Safety Data Sheet

References

Structure, evolutionary conservation, and conformational dynamics of homo sapiens fascin-1, an f-actin crosslinking protein.
Sedeh RS, Fedorov AA, Fedorov EV, Ono S, Matsumura F, Almo SC, Bathe M
J.Mol.Biol.(2010)400p.589
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