References
Fascin (Fascin 1)(Actin Binding Protein)Cat. #: 8411-01
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Description
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Protein | Fascin (Synonyms: Fascin 1,55 kDa actin-bundling protein,Singed-like protein,p55) |
Origin | human recombinant - untagged. |
Molecular mass | 55kDa |
Protein description | Fascin is a monomeric actin bundling protein witha molecular mass of 55kDa. |
Actin interaction | Ca++-insensitive actin bundling protein. |
Properties
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Form | Lyophilized, ready-to-use. |
Quantity per unit | 2x50µg |
Buffer | 150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted with 50 µlultrapure water to obtain a 1.0 mg/ml solution. |
Purity &Activity | Purity: >99%by scanning densitometryfrom Coomassie G-250 stained SDS-Gels. Bundling activity: 2µMAtto565-Actin(Cat.#:8162-01) was incubated with Fascin at a molar ratio of 1:10 and 1:4.1 to verify bundling by fluorecence microscopy. All proteins were used as supplied, i.e. without dialysis. The actin stock solution was pre-spun 15min, 15.000xg, 4°C. Low speed cosedimentation assay was performed (20,000xg, 30min) with rabbit skeletal muscleactin(Cat.#:8101-03)in the presence of sugars. At a molar ratio of 4. : 1 (actin : fascin), fascin was mixed with F-actin. After 30min at RT, the samples were used for low speed spinning. SDS-PAGE showed, that fascin was completely pelleted with F-actin. |
Purification notes | Fascinprotein was amplified from human macrophage cDNA and expressed as a GST-tag fusion protein in E. coli. After purification by GST-Agarose and subsequent GST-tag cleavage, the pure protein was yielded by gel filtration chromatography. |
Protein concentration | Determinedby OD280 (0.1%=1.224). |
Storage instructions | Fascin isstored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilizedproteinis kept on ice and should be stored at -20°C in glycerol orCryoProtect(Cat.#: 6011-01).Avoid repeated freeze / thaw cycles. |
Shipping conditions | At ambient temperature. Upon delivery store at -70°C. |
Remarks | |
CAS no. | |
Further Information
References
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn