Filamin(Actin Binding Protein)Cat. #: 8312-01
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Description
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Protein | Filamin (Filamin A) |
Origin | Smooth muscle, turkey |
Molecular mass | 546kDa |
Protein description | Filamin from turkey gizzard is a homodimer (2x273kDa) with a molecular mass of 546kDa, consisting of 24 repeats of ~96 amino acids and an N-terminal actin binding sequence. Filamin crosslinks actin filaments into orthogonal networks.The C-terminal end of each Filamin monomer is characterized by a hinge region, responsible for the flexibility of actin networks. |
Actin interaction | Actin cross linking protein. Kd actin-binding = 0.5µM. |
Properties
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Form | Lyophilized, ready-to-use. |
Quantity per unit | 2x100µg |
Buffer | 10mMNaCl, 20mM Tris pH 7.4, 0.5mM DTT and 2.4% disaccharides, when reconstituted with 100 µlultrapure water to obtain a 1mg/ml solution. |
Purity | >98%by scanning densitometryfrom Coomassie G-250 stained SDS-Gels. |
Purification notes | Purified from turkey gizzard muscle. |
Protein concentration | Determinedby OD280 (0.1%=0.64). |
Storage instructions | Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles. |
Shipping conditions | At ambient temperature. Upon delivery store at -70°C. |
Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. | |
Further Information
ReferencesReal-timedynamicsofemergingactinnetworksincell-mimickingcompartments.Deshpande S, Pfohl T.; PLoS One. 2015 Mar 18;10(3):e0116521. doi: 10.1371/journal.pone.0116521. eCollection 2015.
A high molecular mass protein isolated from chicken gizzard: its localization at the dense plaques and dense bodies of smooth muscle and the Z-disks of skeletal muscle.Terasaki AG, Nakagawa H, Kotani E, Mori H, Ohashi K.,J Cell Sci. 1995 Mar;108 ( Pt 3):857-68
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