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Cofilin(Actin Binding Protein)Cat. #: 8419-02.....................................................................................................................................................................................................................................................................................................................................................................................................................................................
Description
| |
Protein | Cofilin (synonym: n-cofilin , cofilin-1) |
Origin | non-muscle isoform, murine recombinant (untagged) |
Molecular mass | 18.5kDa |
Protein description | Cofilin depolymerizes actin filaments and inhibits polymerization of G-actinin a pH-sensitive manner. It is a regulatory protein of cytoskeletal actin dynamics. Cofilin plays a role in the regulation of cell morphology and cytoskeletal organization. |
Actin interaction | Non-muscle cofilin binds to G- and F-actin in a 1:1 molar ratio. |
Properties
| |
Form | Lyophilized, ready-to-use. |
Quantity per unit | 1x1 mg |
Buffer | 150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted with 1 ml ultrapure water to obtain a 1.0 mg/ml solution. |
Purity | >96%by scanning densitometryfrom Coomassie G-250 stained SDS-Gels. |
Purification notes | Cofilin was expressed as a GST-tag fusion protein in E. coli. After purification by GST-Agarose and subsequent GST-tag cleavage, the pure protein was yielded by gel filtration chromatography. |
Protein concentration | Determinedby OD280 (0.1%=0.778) |
Storage instructions | Cofilin isstored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilizedproteinis kept on ice and should be stored at -20°C in glycerol orCryoProtect(Cat.#: 6011-01). Avoid repeated freeze / thaw cycles. |
Shipping conditions | At ambient temperature. Upon delivery store at -70°C. |
Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. | |
Further Information
Product DataSheet
Material and Safety Data Sheet
Protein Sequence on NCBI (human non muscle)
Protein Structures on RCSB (human non muscle)References
InitiationofDNAreplicationrequiresactindynamicsandforminactivity.Parisis N, Krasinska L, Harker B, Urbach S, Rossignol M, Camasses A, Dewar J, Morin N, Fisher D.EMBO J. 2017 Nov 2;36(21):3212-3231. doi: 10.15252/embj.201796585. Epub 2017 Oct 5.
Solution structure of human cofilin: actin binding, pH sensitivity, and relationship to actin-depolymerizing factor.
Pope BJ, Zierler-Gould KM, Kühne R, Weeds AG, Ball LJ.
J Biol Chem. 2004 Feb 6;279(6):4840-8. Epub 2003 Nov 18.
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