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hypermol/Ezrin (human, recombinant) - 2x50 µg/8462-01

Description

Ezrin(Actin Binding Protein)Cat. #: 8462-01...............................................................................................................................................................................

Description

Protein

Ezrin (synonym: villin-2)

Origin

human, recombinant (untagged)

Molecular mass

70kDa

Protein description

Ezrin is probably participating in connecting the cytoskeleton to the plasma membrane. Like many proteins, Ezrin is autoinhibited by a head-tail interaction.

Actin interaction

The C-ter binds to actin, PIP2 and membrane proteins, e.g. CD44.

Properties

Form

Lyophilized, ready-to-use.

Quantity per unit

2x50µg

Buffer

100mM KCl, 20mM Hepes pH 7.4, 0.2mM DTT and 5% sucrose, when reconstituted with 50 µl ultrapure water to obtain a 1.0 mg/ml solution.

Purity

>96%by scanning densitometryfrom Coomassie G-250 stained SDS-Gels.

Purification notes

Ezrin was expressed as a GST-tag fusion protein in E. coli. After purification by GST-Agarose and subsequent GST-tag cleavage, the pure protein was yielded by gel filtration chromatography.

Protein concentration

Determinedby OD280 (0.1%=0.963)

Storage instructions

Ezrin isstored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilizedproteinis kept on ice and should be stored at -20°C in glycerol orCryoProtect(Cat.#: 6011-01). Avoid repeated freeze / thaw cycles.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

Further Information

Product DataSheet

Material and Safety Data Sheet

Protein Sequence on NCBI

ReferencesDisorazolesBlockGroupAStreptococcalInvasionintoEpithelialCellsViaInterferencewith theHostFactorEzrin.

Rox K, Rohde M, Chhatwal GS, Müller R.

Cell Chem Biol. 2017 Feb 16;24(2):159-170. doi: 10.1016/j.chembiol.2016.12.011. Epub 2017 Jan 12.

Solutionstructureofhumancofilin:actinbinding,pHsensitivity, andrelationshiptoactin-depolymerizingfactor.

Pope BJ, Zierler-Gould KM, Kühne R, Weeds AG, Ball LJ.

J Biol Chem. 2004 Feb 6;279(6):4840-8. Epub 2003 Nov 18.

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