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hypermol/CytoStaining Kit 594-Phalloidin/C8815-01

Description

CytoStaining Kit594-PhalloidinSuited for cultured cells & cell extractsCat. #: C8815-01

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Applications

Rapid staining of actin filaments with highly photostable 594-Phalloidin. Suited for cultured cells & cell extracts.

Description

Product

594-Phalloidin (Atto594 phalloidin)

Origin

Amanita PhalloidesConjugate

Molecular mass

N/A

Product description

Rapid staining of single actin filaments with highly photostable 594-Phalloidin. Phalloidin is a bicyclic peptide of a family of toxins isolated from the Amanita phalloides “death cap” mushroom. The peptide is commonly used as a selective stain or counterstain to specifically label F-actin in fixed or permeabilized cells, and cell-free experiments. Labeled phalloidin conjugates bind in a stoichiometric ratio of about one phallotoxin per actin subunit in both muscle and non-muscle cells, and have similar affinity for both large and small filaments. Phalloidins do not bind to monomeric actin and have a stabilizing effect on F-actin.

594-Phalloidin has a strong orange-red fluorescence of high brightness and superior photostability.

Actin interaction

Selective staining of F-actin in fixed and permeabilized cells and cell free F-actin.

Properties

Form

Dry

Quantity

1x 100 Units (sufficient for 100 slides)

Buffer

Reconstitute in 50µl MeOH, DMSO or DMF. Dilute 5µl of the phalloidin stock with 500µl PBS and apply to stain the sample.

Sub cellular localization

Actin, Cytoskeleton

Excitation/Emission

ex 601 nm, em 627 nm

Kit Content

1x 100 Units 594-Phalloidin1x PBS tablet to make 100ml PBS1.0 ml FluoProtect

Storage instructions

Upon reconstitution the product is storedat-5 to -70°C. Always protect from light.

Shipping conditions

At ambient temperature. Upon delivery store at -5 to -70°C.

Remarks

CAS no.

N/A

Further Information

Table of Dyes and Light Sources
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