CytoStaining Kit594-PhalloidinSuited for cultured cells & cell extractsCat. #: C8815-01
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Applications
Rapid staining of actin filaments with highly photostable 594-Phalloidin. Suited for cultured cells & cell extracts.
Description
Product | 594-Phalloidin (Atto594 phalloidin) |
Origin | Amanita PhalloidesConjugate |
Molecular mass | N/A |
Product description | Rapid staining of single actin filaments with highly photostable 594-Phalloidin. Phalloidin is a bicyclic peptide of a family of toxins isolated from the Amanita phalloides “death cap” mushroom. The peptide is commonly used as a selective stain or counterstain to specifically label F-actin in fixed or permeabilized cells, and cell-free experiments. Labeled phalloidin conjugates bind in a stoichiometric ratio of about one phallotoxin per actin subunit in both muscle and non-muscle cells, and have similar affinity for both large and small filaments. Phalloidins do not bind to monomeric actin and have a stabilizing effect on F-actin. 594-Phalloidin has a strong orange-red fluorescence of high brightness and superior photostability. |
Actin interaction | Selective staining of F-actin in fixed and permeabilized cells and cell free F-actin. |
Properties
Form | Dry |
Quantity | 1x 100 Units (sufficient for 100 slides) |
Buffer | Reconstitute in 50µl MeOH, DMSO or DMF. Dilute 5µl of the phalloidin stock with 500µl PBS and apply to stain the sample. |
Sub cellular localization | Actin, Cytoskeleton |
Excitation/Emission | ex 601 nm, em 627 nm |
Kit Content | 1x 100 Units 594-Phalloidin1x PBS tablet to make 100ml PBS1.0 ml FluoProtect |
Storage instructions | Upon reconstitution the product is storedat-5 to -70°C. Always protect from light. |
Shipping conditions | At ambient temperature. Upon delivery store at -5 to -70°C. |
Remarks | |
CAS no. | N/A |
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