AnnexinVfluorescentconjugatescanbeusedtodetectapoptoticcells.ThehumananticoagulantAnnexinVisa35-36kilodalton, calcium-dependentphospholipid-bindingproteinwithhighaffinityforphosphatidylserine(PS).Innormalviablecells,PSislocatedontheinnerleafletofthecytoplasmicmembrane.Inapoptoticcells,however,PSistranslocatedfromtheinnertotheouterleafletoftheplasmamembrane,whereitcanbedetectedbyfluorescentlylabeledAnnexinV.
It’savailablewiththefollowingdyes: CF™350, CF™405M,CF™488A,CF™555,CF™568,CF™594,CF™633,CF™640R,CF™647,CF™660R,CF™680R,FITC,Sulforhodamine101(TexasRed®),Biotin,R-PE,andAPC.
AlsoseeourNear-IRCF™DyeAnnexinVConjugates,suppliedlyophilizedandpreservative-free.
WealsoofferAnnexinVinthefollowingapoptosisassaykits:
TexasRedisaregisteredtrademarkofThermoFisherScientific.
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2.Blood84,1415(1994).
3.Cytometry43,134(2001).
4.FEBSLett.203,99(1986).
5.Biosci.Rep.7,289(1987).
DownloadalistofCFdyereferences.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn