ATP-Glo™BioluminometricCellViabilityAssayoffersahighlysensitiveassayforquantifyingATP.ThehomogeneousassayprocedureinvolvesasingleadditionofATP-Glo™DetectionCocktaildirectlytocellsculturedinaserum-supplementedmedium.Itisnotnecessarytoremovecellculturemediumorwashcellsbeforeaddingthereagent.
BecauseATPisanindicatorofmetabolicallyactivecells,thenumberofviablecellscanbeassessedbasedontheamountofATPavailable.ThisATPdetectionkittakesadvantageoffireflyluciferase’suseofATPtooxidizeD-LuciferinandtheresultingproductionoflightinordertoassesstheamountofATPavailable.
TheATP-Glo™kitcanbeusedtodetectaslittleasasinglecellor0.01picomoleofATP.Thesignalproducedislinearwithin6ordersofmagnitude.ByrelatingtheamountofATPtothenumberofviablecells,theassayhaswideapplications,rangingfromthedeterminationofviablecellnumberstocellproliferationtocellcytotoxicity.
Pleasenote:ATP-Glo™isaflash-typeluminescenceassay.Theluminescencesignalgeneratedisstableforupto1minute.Thisassayisdesignedforindividualsampledetectionbyusingaluminometerinasinglesampleformatoraluminometerwithaninjectorin96-wellplateformat.
KitComponents:
Features:
MoreLuciferaseKits
Catalognumber | Product | Description |
---|---|---|
30085 | FireflyLuciferaseAssayKit2.0 | Flash-typefireflyluciferaseassaykit. |
30075 | FireflyLuciferaseAssayKit(Lyophilized) | Fireflyluciferaseassaykitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C. |
99923 | 5XFireflyLysisBuffer | ExtralysisbufferforusewithFireflyLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well. |
30028 | Steady-LucFireflyHTSAssayKit | Glow-typefireflyluciferaseassaywithsignalhalf-lifeofabout3hoursforhigh-throughputscreeninginmulti-wellplates. |
30028L | Steady-LucFireflyHTSAssayKit(Lyophilized) | Steady-Luckitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C. |
30082 | RenillaLuciferaseAssayKit2.0 | Flash-typeRenillaluciferaseassaykit. |
99912 | 5XPassiveLysisBuffer | ExtralysisbufferforusewithRenillaLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well. |
30081 | FireflyandRenillaLuciferaseSingleTubeAssayKit | AssaykitformeasuringfireflyandRenillaactivitiessequentiallyinthesamesampleinasingletube. |
99821-100mL | 1XPassiveLysisBuffer2.0 | ExtralysisbufferforusewithFirefly&RenillaSingleTubeAssayKits,onlyrequiredifusingwellsizeslargerthan24-well. |
30020 | ATP-GloBioluminometricCellViabilityAssayKit | Flash-typeluminescence-basedassayformeasuringcellviabilityorATPconcentration. |
22003 | MiniCellScrapers | Mini-cellscrapers,usefulforlysingcellsin96-well,48-well,or24-wellplates. |
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ImmunolMeth160,81(1993).
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